Please wait a minute...
J Integr Plant Biol, 2015, 57 (4): 341-348, Research Article
Norway spruce (Picea abies) laccases: Characterization of a laccase in a lignin-forming tissue culture
Sanna Koutaniemi1, Heli A. Malmberg1†, Liisa K. Simola2‡, Teemu H. Teeri1 and Anna Kärkönen1*
1Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland
2Department of Biosciences, University of Helsinki, Helsinki, Finland
These authors contributed equally to this work.
Present address: Ritarikatu 9 B, Helsinki 00170, Finland.
*Correspondence: E-mail: anna.karkonen@helsinki.fi
doi: 10.1111/jipb.12333
Abstract

Secondarily thickened cell walls of water-conducting vessels and tracheids and support-giving sclerenchyma cells contain lignin that makes the cell walls water impermeable and strong. To what extent laccases and peroxidases contribute to lignin biosynthesis in muro is under active evaluation. We performed an in silico study of Norway spruce (Picea abies (L.) Karst.) laccases utilizing available genomic data. As many as 292 laccase encoding sequences (genes, gene fragments, and pseudogenes) were detected in the spruce genome. Out of the 112 genes annotated as laccases, 79 are expressed at some level. We isolated five full-length laccase cDNAs from developing xylem and an extracellular lignin-forming cell culture of spruce. In addition, we purified and biochemically characterized one culture medium laccase from the lignin-forming cell culture. This laccase has an acidic pH optimum (pH 3.8–4.2) for coniferyl alcohol oxidation. It has a high affinity to coniferyl alcohol with an apparent Km value of 3.5 μM; however, the laccase has a lower catalytic efficiency (Vmax/Km) for coniferyl alcohol oxidation compared with some purified culture medium peroxidases. The properties are discussed in the context of the information already known about laccases/coniferyl alcohol oxidases of coniferous plants.

 

Koutaniemi S, Malmberg HA, Simola LK, Teeri TH, Kärkönen A (2015) Norway spruce (Picea abies) laccases: Characterization of a laccase in a lignin-forming tissue culture. J Integr Plant Biol 57: 341–348 doi: 10.1111/jipb.12333

--------------------------------------------------------------------------------------------------------------------
Received: 05 November 2014      Accepted:    Online on:
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
   Articles by Sanna Koutaniemi
   Articles by Heli A. Malmberg
   Articles by Liisa K. Simola
   Articles by Teemu H. Teeri
   Articles by Anna KÄrkönen
PubMed
   Articles by Sanna Koutaniemi
   Articles by Heli A. Malmberg
   Articles by Liisa K. Simola
   Articles by Teemu H. Teeri
   Articles by Anna KÄrkönen
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22