J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (9): 834-839.DOI: 10.1111/j.1744-7909.2009.00864.x

• Cell and Developmental Biology • Previous Articles     Next Articles

Variation of B Chromosome Associated with Tissue Culture in Wheat-rye Cross

Bohong Tian1 and Hongjie Li2*   

  1. 1 Cangzhou Academy of Agricultural and Forestry Sciences, Cangzhou 061001, China
    2 The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2008-11-04 Accepted:2009-07-07 Published:2009-08-31
  • About author: *Author for correspondence Tel: +86 10 8210 9609; Fax: +86 10 8210 9608; E-mail: hongjie@caas.net.cn

Abstract:

In vitro variation of B chromosomes was studied by examining the callus cells derived from the immature embryos from a cross of Chinese Spring wheat (Triticum aestivum L.) and Fin 7416 rye (Secale cereale L.) carrying two B chromosomes. In 40-d-old callus cells, the numbers of B chromosomes ranged from one to four in 65.6% of the cells observed. The distribution of B chromosome numbers was associated with the ploidy levels of the normal chromosomes (A chromosomes). The frequency of the cells with high numbers of B chromosomes (i.e., three or four B chromosomes) in the amphiploid cells with 56 A chromosomes was greater than those in the haploid cells with 28 A chromosomes. Although structural changes in the rye A chromosomes were observed, cytological observation and genomic in situ hybridization demonstrated that the rye B chromosomes were conserved in morphological appearance following tissue culture.

Tian B, Li H (2009). Variation of B chromosome associated with tissue culture in wheat-rye cross. J. Integr. Plant Biol. 51(9), 834–839.

Key words: amphiploid, B chromosome, genomic in situ,/em> hybridization, Secale cereale, tissue culture, Triticum aestivum.

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