J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (1): 12-28.DOI: 10.1111/jipb.12372

Special Issue: Rice Genomics and Agriculture

• Functional Omics and Systems Biology • Previous Articles     Next Articles

Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice

Qi-Na Huang1, Yong-Feng Shi1, Xiao-Bo Zhang1, Li-Xin Song1,2, Bao-Hua Feng1, Hui-Mei Wang1, Xia Xu1, Xiao-Hong Li1, Dan Guo1 and Jian-Li Wu1*   

  1. 1State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China
    2School of Life Sciences, Hebei Normal University, Shijiazhuang, China
  • Received:2015-05-05 Accepted:2015-05-19 Published:2015-06-04
  • About author:*Correspondence: E-mail: beishangd@163.com

Abstract:

A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate-induced rice IR64 mutant bank. The premature senescence phenotype appeared at the six-leaf stage and the plant died at the early heading stage. psd128 exhibited impaired chloroplast development with significantly reduced photosynthetic ability, chlorophyll and carotenoid contents, root vigor, soluble protein content and increased malonaldehyde content. Furthermore, the expression of senescence-related genes was significantly altered in psd128. The mutant trait was controlled by a single recessive nuclear gene. Using map-based strategy, the mutation Oryza sativa cell division cycle 48 (OsCDC48) was isolated and predicted to encode a putative AAA-type ATPase with 809 amino-acid residuals. A single base substitution at position C2347T in psd128 resulted in a premature stop codon. Functional complementation could rescue the mutant phenotype. In addition, RNA interference resulted in the premature senescence and death phenotype. OsCDC48 was expressed constitutively in the root, stem, leaf and panicle. Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus. OsCDC48 was highly conserved with more than 90% identity in the protein levels among plant species. Our results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype.

 

Huang QN, Shi YF, Zhang XB, Song LX, Feng BH, Wang HM, Xu X, Li XH, Guo D, Wu JL (2016) Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice. J Integr Plant Biol 58: 12–28 doi: 10.1111/jipb.12372

Key words: Oryza sativa, Premature senescence and death, AAA-ATPase, OsCDC48

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