J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (10): 819-829.DOI: 10.1111/jipb.12340

Special Issue: Rice Genomics and Agriculture

• Cell and Developmental Biology • Previous Articles     Next Articles

The alteration in the architecture of a T-DNA insertion rice mutant osmtd1 is caused by up-regulation of MicroRNA156f

Qing Liu1, Gezhi Shen2, Keqin Peng1, Zhigang Huang1, Jianhua Tong1, Mohammed Humayun Kabir1, Jianhui Wang3, Jingzhe Zhang4, Genji Qin4* and Langtao Xiao1*   

  1. 1Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Provincial Key Laboratory for Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, China
    2Crop Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
    3Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China
    4State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, China

Abstract:

Plant architecture is an important factor for crop production. Some members of microRNA156 (miR156) and their target genes SQUAMOSA Promoter-Binding Protein-Like (SPL) were identified to play essential roles in the establishment of plant architecture. However, the roles and regulation of miR156 is not well understood yet. Here, we identified a T-DNA insertion mutant Osmtd1 (Oryza sativa multi-tillering and dwarf mutant). Osmtd1 produced more tillers and displayed short stature phenotype. We determined that the dramatic morphological changes were caused by a single T-DNA insertion in Osmtd1. Further analysis revealed that the T-DNA insertion was located in the gene Os08g34258 encoding a putative inhibitor I family protein. Os08g34258 was knocked out and OsmiR156f was significantly upregulated in Osmtd1. Overexpression of Os08g34258 in Osmtd1 complemented the defects of the mutant architecture, while overexpression of OsmiR156f in wild-type rice phenocopied Osmtd1. We showed that the expression of OsSPL3, OsSPL12, and OsSPL14 were significantly downregulated in Osmtd1 or OsmiR156f overexpressed lines, indicating that OsSPL3, OsSPL12, and OsSPL14 were possibly direct target genes of OsmiR156f. Our results suggested that OsmiR156f controlled plant architecture by mediating plant stature and tiller outgrowth and may be regulated by an unknown protease inhibitor I family protein.

 

Liu Q, Shen G, Peng K, Huang Z, Tong J, Kabir MH, Wang J, Zhang J, Qin G, Xiao L (2015) The alteration in the architecture of a T-DNA insertion rice mutant osmtd1 is caused by up-regulation of MicroRNA156f. J Integr Plant Biol 57: 819–829 doi: 10.1111/jipb.12340

Key words: Oryza sativa, OsmiR156f, plant architecture, protease inhibitor, T-DNA insertion

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