J Integr Plant Biol. ›› 2016, Vol. 58 ›› Issue (9): 766-771.DOI: 10.1111/jipb.12472

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An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber

Xin Wang1,2,†, Dongli Gao2,3,†, Jinjing Sun2, Min Liu2, Yaoyao Lun2, Jianshu Zheng3, Shenhao Wang2, Qingzhi Cui4, Xiaofeng Wang1* and Sanwen Huang1,2*   

  1. 1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, China
    2Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
    4Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China


We isolated a mutant showing perturbations in the development of male and female floral organs and fruits. Analysis of the single nucleotide polymorphisms from bulked F2 pools identified the causative variant occurring in Csa4G126690. Csa4G126690 shows high homology to Arabidopsis SEPALLATA2 (SEP2) thus being designated CsSEP2. The causative variant was located on the splicing site of CsSEP2, resulting in the skipping of exon 6 and abolishment of the transcriptional activity. Our data suggest that CsSEP2 is involved in the floral organ and fruits development by conferring transcriptional activity.

Key words: Cucumis sativus L, EMS mutant, exon skipping, floral and fruits development, whole genome re-sequencing

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