J Integr Plant Biol. ›› 2018, Vol. 60 ›› Issue (2): 144-159.DOI: 10.1111/jipb.12603

Special Issue: Rice Genomics and Agriculture

• Research Articles • Previous Articles     Next Articles

Chromatin-remodeling factor OsINO80 is involved in regulation of gibberellin biosynthesis and is crucial for rice plant growth and development

Chao Li1, Yuhao Liu1, Wen-Hui Shen1,2, Yu Yu1* and Aiwu Dong1*   

  1. 1State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China
    2Institut de Biologie Moléculaire des Plantes, UPR2357 CNRS, Université de Strasbourg, 67084 Strasbourg Cédex, France
  • Received:2017-09-27 Accepted:2017-10-17 Published:2018-02-13
  • About author:**Correspondences: Email: Aiwu Dong (aiwudong@fudan.edu.cn, Dr. Dong is fully responsible for the distribution of all materials associated with this article); yuy@fudan.edu.cn

Abstract:

The phytohormone gibberellin (GA) plays essential roles in plant growth and development. Here, we report that OsINO80, a conserved ATP-dependent chromatin-remodeling factor in rice (Oryza sativa), functions in both GA biosynthesis and diverse biological processes. OsINO80-knockdown mutants, derived from either T-DNA insertion or RNA interference, display typical GA-deficient phenotypes, including dwarfism, reduced cell length, late flowering, retarded seed germination and impaired reproductive development. Consistently, transcriptome analyses reveal that OsINO80 knockdown results in downregulation by more than two-fold of over 1,000 genes, including the GA biosynthesis genes CPS1 and GA3ox2, and the dwarf phenotype of OsINO80-knockdown mutants can be rescued by the application of exogenous GA3. Chromatin immunoprecipitation (ChIP) experiments show that OsINO80 directly binds to the chromatin of CPS1 and GA3ox2 loci. Biochemical assays establish that OsINO80 specially interacts with histone variant H2A.Z and the H2A.Z enrichments at CPS1 and GA3ox2 are decreased in OsINO80-knockdown mutants. Thus, our study identified a rice chromatin-remodeling factor, OsINO80, and demonstrated that OsINO80 is involved in regulation of the GA biosynthesis pathway and plays critical functions for many aspects of rice plant growth and development.

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