J Integr Plant Biol ›› 2018, Vol. 60 ›› Issue (7): 591-607.DOI: 10.1111/jipb.12649

Special Issue: Plant Signal Transduction CRISPR

• Molecular Physiology • Previous Articles     Next Articles

The Arabidopsis catalase triple mutant reveals important roles of catalases and peroxisome‐derived signaling in plant development

Tong Su1†, Pingping Wang1†, Huijuan Li1†, Yiwu Zhao1, Yao Lu1, Peng Dai1, Tianqi Ren1, Xiaofeng Wang1, Xuezhi Li1, Qun Shao1, Dazhong Zhao2, Yanxiu Zhao1* and Changle Ma1*   

  1. 1Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Wenhua East Road 88, Jinan250014, China
    2Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA
  • Received:2018-02-10 Accepted:2018-03-19 Published:2018-07-01
  • About author:These authors contributed equally to this work
    **Correspondences: Email: Yanxiu Zhao (zhaoyx@sdnu.edu.cn); Changle Ma (machangle@sdnu.edu.cn, Dr. Ma is fully responsible for the distribution of all materials associated with this article)

Abstract:

Hydrogen peroxide (H2O2) is generated in many metabolic processes. As a signaling molecule, H2O2 plays important roles in plant growth and development, as well as environmental stress response. In Arabidopsis, there are three catalase genes, CAT1, CAT2, and CAT3. The encoded catalases are predominately peroxisomal proteins and are critical for scavenging H2O2. Since CAT1 and CAT3 are linked on chromosome 1, it has been almost impossible to generate cat1/3 and cat1/2/3 mutants by traditional genetic tools. In this study, we constructed cat1/3 double mutants and cat1/2/3 triple mutants by CRISPR/Cas9 to investigate the role of catalases. The cat1/2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditions compared with wild‐type and the cat2/3 double mutants. Transcriptome analysis showed a more profound transcriptional response in the cat1/2/3 triple mutants compared to the cat2/3 mutants. These differentially expressed genes are involved in plant growth regulation as well as abiotic and biotic stress responses. In addition, expression of OXI1 (OXIDATIVE SIGNAL INDUCIBLE 1) and several MAPK cascade genes were changed dramatically in the catalase triple mutant, suggesting that H2O2 produced in peroxisomes could serve as a peroxisomal retrograde signal.

Catalases, which are predominate peroxisomal proteins, function as antioxidant enzymes to scavenge H2O2. In this study, using the CRISPR/Cas9 technology, we generated the cat1/2/3 triple mutants, which displayed severe redox disturbance and growth defects. Transcriptome analysis showed that H2O2 produced in peroxisomes could serve as a peroxisomal retrograde signal.
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