J Integr Plant Biol. ›› 2018, Vol. 60 ›› Issue (8): 626-631.DOI: 10.1111/jipb.12667

Special Issue: Rice Genomics and Agriculture CRISPR

• Letter to the Editor •     Next Articles

Multiplex gene editing in rice with simplified CRISPR‐Cpf1 and CRISPR‐Cas9 systems

Mugui Wang1*, Yanfei Mao1, Yuming Lu1, Zhidan Wang1, Xiaoping Tao1 and Jian-Kang Zhu1,2*   

  1. 1Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China
    2Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA

    Email: Mugui Wang (mgwang@sibs.ac.cn), Jian-Kang Zhu (jkzhu@sibs.ac.cn, Dr. Zhu is fully responsible for the distribution of all materials associated with this article.)
  • Received:2018-03-23 Accepted:2018-05-10 Online:2018-05-15 Published:2018-08-01

Abstract: We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co‐expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.

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