J Integr Plant Biol. ›› 2018, Vol. 60 ›› Issue (12): 1231-1248.DOI: 10.1111/jipb.12705

• Invited Expert Reviews • Previous Articles    

Biotechnological approaches in glucosinolate production

Annette Petersen, Cuiwei Wang, Christoph Crocoll and Barbara Ann Halkier*   

  1. DynaMo Center, Copenhagen Plant Science Centre, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark

    ?These authors contributed equally to this work.

    Email: Barbara Ann Halkier (bah@plen.ku.dk)
  • Received:2018-05-05 Accepted:2018-07-31 Online:2018-08-06 Published:2018-12-01

Abstract: Glucosinolates (GLSs) are sulfur-rich, amino acid-derived defense compounds characteristic of the Brassicales order. In the past, GLSs were mostly known as anti-nutritional factors in fodder, biopesticides in agriculture, and flavors in condiments such as mustard. However, in recent times, GLSs have received increased attention as promoters of human health. This has spurred intensive research towards generating rich sources of health-promoting GLSs. We provide a comprehensive overview of the biotechnological approaches applied to reach this goal. This includes optimization of GLS production and composition in native, GLS-producing plants, including hairy root and cell cultures thereof, as well as synthetic biology approaches in heterologous hosts, such as tobacco and the microbial organisms Escherichia coli and Saccharomyces cerevisiae. The progress using these different approaches is discussed.

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