, Volume 60 Issue 12
Special issue: Plant synthetic biology
The nuclease domain of Cas9 can be deactivated by point mutations, to convert the protein into a sequence‐specific DNA‐binding protein. In this issue, Kumlehn et al. (1127–1153) discuss the use of the resultant deactivated Cas9 protein to target various functions by binding to specific sites in genome for applications, such as imaging, epigenetic and transcriptional regulation, base editing or aptamer fusions.