J Integr Plant Biol. ›› 2019, Vol. 61 ›› Issue (4): 388-393.DOI: 10.1111/jipb.12721

• Letters to the Editor • Previous Articles     Next Articles

Towards a better recording of microtubule cytoskeletal spatial organization and dynamics in plant cells

Weiwei Liu1,2† Chaofeng Wang1,2†, Guangda Wang1,2, Yinping Ma1,2, Juan Tian1, Yanjun Yu1, Li Dong1 and Zhaosheng Kong1,2*   

  1. 1State Key Laboratory of Plant Genomics, Institute of Microbiology, the Chinese Academy of Sciences, Beijing 100101, China
    2The University of Chinese Academy of Sciences, Beijing 100049, China

    ?These authors contributed equally to this work.

    Email: Zhaosheng Kong (zskong@im.ac.cn)
  • Received:2018-07-23 Accepted:2018-09-07 Online:2018-09-18 Published:2019-04-01

Abstract: Numerous fluorescent marker lines are currently available to visualize microtubule (MT) architecture and dynamics in living plant cells, such as markers expressing p35S::GFP-MBD or p35S::GFP-TUB6. However, these MT marker lines display obvious defects that affect plant growth or produce unstable fluorescent signals. Here, a series of new marker lines were developed, including the pTUB6::VisGreen-TUB6-expressing line in which TUB6 is under the control of its endogenous regulatory elements and eGFP is replaced with VisGreen, a brighter fluorescent protein. Moreover, two different markers were combined into one expression vector and developed two dual-marker lines. These marker lines produce bright, stable fluorescent signals in various tissues, and greatly shorten the screening process for generating dual-marker lines. These new marker lines provide a novel resource for MT research.

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