J Integr Plant Biol. ›› 2019, Vol. 61 ›› Issue (8): 943-963.DOI: 10.1111/jipb.12722

Special Issue: Rice Genomics and Agriculture

• Plant Reproduction Biology • Previous Articles    

Brassinosteroids function in spikelet differentiation and degeneration in rice

Weiyang Zhang1,2, Kuanyu Zhu1,2, Zhiqin Wang1,2, Hao Zhang1,2, Junfei Gu1,2, Lijun Liu1,2, Jianchang Yang1,2* and Jianhua Zhang3*   

  1. 1Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China
    2Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China
    3Department of Biology, Hong Kong Baptist University, Hong Kong, China

    *Correspondence:
    Email: Jianchang Yang (jcyang@yzu.edu.cn; Dr. Yang is fully responsible for the distribution of all materials associated with this article); Jianhua Zhang (jzhang@hkbu.edu.hk)
  • Received:2018-07-02 Accepted:2018-09-19 Online:2018-09-24 Published:2019-08-01

Abstract:

Brassinosteroids (BRs) play crucial roles in many aspects of plant development. However, their function in spikelet differentiation and degeneration in rice (Oryza sativa L.) remains unclear. Here, we investigated the roles of these phytohormones in spikelet development in field‐grown rice subjected to five different nitrogen (N) fertilization treatments during panicle differentiation. BR levels and expression of genes involved in BR biosynthesis and signal transduction were measured in spikelets. Pollen fertility and the number of differentiated spikelets were closely associated with 24‐epicastasterone (24‐epiCS) and 28‐homobrassinolide (28‐homoBL) levels in spikelets. Enhanced BR biosynthesis and signal transduction, in response to N treatment, enhanced spikelet differentiation, reduced spikelet degeneration, and increased grain yield. Increases in proton‐pumping ATPase activity, ATP concentration, energy charge, and antioxidant system (AOS) levels were consistent with 24‐epiCS and 28‐homoBL concentrations. Exogenous application of 24‐epiCS or 28‐homoBL on young panicles induced a marked increase in endogenous 24‐epiCS or 28‐homoBL levels, energy charge, AOS levels, spikelet differentiation, and panicle weight. The opposite effects were observed following treatment with a BR biosynthesis inhibitor. Our findings indicate that, in rice, BRs mediate the effects of N fertilization on spikelet development and play a role in promoting spikelet development through increasing AOS levels and energy charge during panicle development.

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