J Integr Plant Biol ›› 2019, Vol. 61 ›› Issue (9): 974-980.DOI: 10.1111/jipb.12728

• Letters to the Editor • Previous Articles     Next Articles

Endoplasmic reticulum stress-induced accumulation of VAMP721/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis

Soohong Kim1, Yunjin Choi1, Chian Kwon1,* and Hye Sup Yun2,*   

  1. 1Department of Molecular Biology, Dankook University, Cheonan 31116, Korea
    2Department of Biological Sciences, Konkuk University, Seoul 05029, Korea

    *Correspondence:
    Email: Hye Sup Yun (hsyun@konkuk.ac.kr); Chian Kwon (chiank@dankook.ac.kr).Drs. Yun and Kwon are fully responsible for the distribution of all materials associated with this article.
  • Received:2018-06-14 Accepted:2018-09-27 Online:2018-10-02 Published:2019-09-01

Abstract:

Excessive demand for translation and protein folding in the endoplasmic reticulum (ER) can cause ER stress in plants. Here, we show that CALRETICULIN 1 (CRT1) and CRT2 are critical components in the accumulation of VESICLE‐ASSOCIATED MEMBRANE PROTEIN 721 (VAMP721) and VAMP722 during ER stress responses. We show that CRT2 interacts with VAMP722 and that CRT1/2 post‐translationally maintain elevated VAMP721/722 levels under ER stress. The greater growth inhibition in VAMP721/722‐deficient plants, induced by tunicamycin, suggests that plants under ER stress maintain physiological homeostasis, at least in part, by regulating VAMP721/722 levels, as VAMP721/722 are known to participate in various biological processes.

VAMP721/722 are the exocytosis‐driving R‐SNAREs, which are required for growth and environmental stress responses in plants. Elevated VAMP721/722 transcript levels but not VAMP721/722 protein levels by tunicamycin in the CRT1/2‐deficient plants, and the interaction between VAMP721/722 and CRT1/2, suggest that CRT1/2 may stabilize VAMP721/722 likely for ER stress responses in plants.
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22