J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (5): 702-715.DOI: 10.1111/jipb.12816

Special Issue: Plant-biotic interaction

• Plant-biotic Interactions • Previous Articles    

Osa‐miR167d facilitates infection of Magnaporthe oryzae in rice

Zhi-Xue Zhao, Qin Feng, Xiao-Long Cao, Yong Zhu, He Wang, Viswanathan Chandran, Jing Fan, Ji-Qun Zhao, Mei Pu, Yan Li and Wen-Ming Wang*   

  1. Rice Research Institute and Key Lab for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China

    Current address: Department of Biotechnology, Sree Narayana Guru College, Coimbatore, Tamilnadu, India
    Email: Wen-Ming Wang (j316wenmingwang@sicau.edu.cn)
  • Received:2019-03-13 Accepted:2019-04-16 Online:2019-04-19 Published:2020-05-01


MicroRNAs (miRNAs) play important roles in rice response to Magnaporthe oryzae, the causative agent of rice blast disease. Studying the roles of rice miRNAs is of great significance for the disease control. Osa‐miR167d belongs to a conserved miRNA family targeting auxin responsive factor (ARF) genes that act in developmental and stress‐induced responses. Here, we show that Osa‐miR167d plays a negative role in rice immunity against M. oryzae by suppressing its target gene. The expression of Osa‐miR167d was significantly suppressed in a resistant accession at and after 24 h post inoculation (hpi), however, its expression was significantly increased at 24 hpi in the susceptible accession upon M. oryzae infection. Transgenic rice lines over‐expressing Osa‐miR167d were highly susceptible to multiple blast fungal strains. By contrast, transgenic lines expressing a target mimicry to block Osa‐miR167d enhanced resistance to rice blast disease. In addition, knocking out the target gene ARF12 led to hyper‐susceptibility to multiple blast fungal strains. Taken together, our results indicate that Osa‐miR167d negatively regulate rice immunity to facilitate the infection of M. oryzae by downregulating ARF12. Thus, Osa‐miR167d‐ARF12 regulatory module could be valuable in improvement of blast‐disease resistance.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q