J Integr Plant Biol ›› 2020, Vol. 62 ›› Issue (5): 702-715.DOI: 10.1111/jipb.12816

• Plant-biotic Interactions • Previous Articles    

Osa‐miR167d facilitates infection of Magnaporthe oryzae in rice

Zhi-Xue Zhao, Qin Feng, Xiao-Long Cao, Yong Zhu, He Wang, Viswanathan Chandran, Jing Fan, Ji-Qun Zhao, Mei Pu, Yan Li and Wen-Ming Wang*   

  1. Rice Research Institute and Key Lab for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, China

    Current address: Department of Biotechnology, Sree Narayana Guru College, Coimbatore, Tamilnadu, India
    *
    Correspondence:
    Email: Wen-Ming Wang (j316wenmingwang@sicau.edu.cn)
  • Received:2019-03-13 Accepted:2019-04-16 Online:2019-04-19 Published:2020-05-01

Abstract:

MicroRNAs (miRNAs) play important roles in rice response to Magnaporthe oryzae, the causative agent of rice blast disease. Studying the roles of rice miRNAs is of great significance for the disease control. Osa‐miR167d belongs to a conserved miRNA family targeting auxin responsive factor (ARF) genes that act in developmental and stress‐induced responses. Here, we show that Osa‐miR167d plays a negative role in rice immunity against M. oryzae by suppressing its target gene. The expression of Osa‐miR167d was significantly suppressed in a resistant accession at and after 24 h post inoculation (hpi), however, its expression was significantly increased at 24 hpi in the susceptible accession upon M. oryzae infection. Transgenic rice lines over‐expressing Osa‐miR167d were highly susceptible to multiple blast fungal strains. By contrast, transgenic lines expressing a target mimicry to block Osa‐miR167d enhanced resistance to rice blast disease. In addition, knocking out the target gene ARF12 led to hyper‐susceptibility to multiple blast fungal strains. Taken together, our results indicate that Osa‐miR167d negatively regulate rice immunity to facilitate the infection of M. oryzae by downregulating ARF12. Thus, Osa‐miR167d‐ARF12 regulatory module could be valuable in improvement of blast‐disease resistance.

Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases. Here we demonstrated that rice Osa-miR167d facilitates the infection of M. oryzae by suppressing ARF12, and ARF12 is required for resistance to the blast disease. Our findings suggest that the Osa-miR167d-ARF12 regulatory module is valuable in blast-disease breeding programs.
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