J Integr Plant Biol ›› 2020, Vol. 62 ›› Issue (5): 601-613.DOI: 10.1111/jipb.12821

• Metabolism and Biochemistry • Previous Articles     Next Articles

Arabidopsis ADC1 functions as an Nδ‐acetylornithine decarboxylase

Yann‐Ru Lou1†, Sheaza Ahmed2, Jian Yan3, Adewale M. Adio1‡, Hannah M. Powell1, Paul F. Morris2 and Georg Jander1*   

  1. 1Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA
    2Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403, USA
    3Key Laboratory of Agro‐Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China

    Present address: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA
    Present address: Bayer Crop Science, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
    *Correspondence:
    Email: Georg Jander(gj32@cornell.edu)
  • Received:2019-03-23 Accepted:2019-04-28 Online:2019-05-13 Published:2020-05-01

Abstract:

Polyamines are small aliphatic amines found in almost all organisms, ranging from bacteria to plants and animals. In most plants, putrescine, the metabolic precursor for longer polyamines, such as spermidine and spermine, is produced from arginine, with either agmatine or ornithine as intermediates. Here we show that Arabidopsis thaliana (Arabidopsis) arginine decarboxylase 1 (ADC1), one of the two known arginine decarboxylases in Arabidopsis, not only synthesizes agmatine from arginine, but also converts Nδacetylornithine to N‐acetylputrescine. Phylogenetic analyses indicate that duplication and neofunctionalization of ADC1 and NATA1, the enzymes that synthesize Nδacetylornithine in Arabidopsis, co‐occur in a small number of related species in the Brassicaceae. Unlike ADC2, which is localized in the chloroplasts, ADC1 is in the endoplasmic reticulum together with NATA1, an indication that these two enzymes have access to the same substrate pool. Together, these results are consistent with a model whereby NATA1 and ADC1 together provide a pathway for the synthesis of N‐acetylputrescine in Arabidopsis.

An arginine decarboxylase in Arabidopsis was shown to function as an N‐delta‐acetylornithine decarboxylase as well, leading to the formation of N‐acetylputrescine. This enzymatic activity provides a route for the formation of acetylated polyamines. Polyamine acetylation may facilitate transport across membranes and function in plant stress responses.
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22