J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (7): 1034-1056.DOI: 10.1111/jipb.12871

Special Issue: Development

• Plant Reproduction Biology • Previous Articles    

The transcription factor GATA10 regulates fertility conversion of a two-line hybrid tms5 mutant rice via the modulation of Ub L40 expression

Jing Jin1, Songtao Gui1, Qian Li1, Ying Wang1, Hongyuan Zhang2, Zhixuan Zhu1, Hao Chen1, Yueyang Sun1, Yu Zou1, Xingguo Huang3 and Yi Ding1*   

  1. 1State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China
    2Institute of Vegetable, Wuhan Academy of Agricultural Sciences, Wuhan 430072, China
    3Wuhan Wuda Tianyuau Bio-Tech Co., Ltd., Wuhan 430070, China

    Email: Yi Ding (yiding@whu.edu.cn)
  • Received:2019-07-08 Accepted:2019-08-30 Online:2019-09-06 Published:2020-07-01


The thermosensitive genic male sterile 5 (tms5 ) mutation causes thermosensitive genic male sterility in rice (Oryza sativa ) through loss of RNase ZS1 function, which influences ubiquitin fusion ribosomal protein L40 (Ub L40 ) messenger RNA levels during male development. Here, we used ATAC‐seq, combined with analysis of H3K9ac and H3K4me2, to identify changes in accessible chromatin during fertility conversion of the two‐line hybrid rice Wuxiang S (WXS) derived from a mutant tms5 allele. Furthermore, RNA‐seq and bioinformatic analyses identified specific transcription factors (TFs) in differentially accessible chromatin regions. Among these TFs, only GATA10 targeted Ub L40 . Osgata10 knockout mutations, which resulted in low expression of Ub L40 and a tendency toward male fertility, confirmed that GATA10 regulated fertility conversion via the modulation of Ub L40 . Meanwhile, GATA10 acted as a mediator for interactions with ERF65, which revealed that transcriptional regulation is a complex process involving multiple complexes of TFs, namely TF modules. It appears that the ERF141/MADS7/MADS50/MYB modules affect metabolic processes that control anther and pollen development, especially cell wall formation. Our analysis revealed that these modules directly or indirectly affect metabolic pathway‐related genes to coordinate plant growth with proper anther development, and furthermore, that GATA10 regulates fertility conversion via the modulation of Ub L40 expression.

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