J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (8): 1227-1245.DOI: 10.1111/jipb.12897

Special Issue: Development

• Plant Reproduction Biology • Previous Articles     Next Articles

Lectin receptor kinase OsLecRK-S.7 is required for pollen development and male fertility

Xiaoqun Peng1,2†, Menglong Wang1,2†, Yiqi Li1, Wei Yan1,2, Zhenyi Chang1, Zhufeng Chen2, Chunjue Xu2, Chengwei Yang1, Xing Wang Deng2,3, Jianxin Wu1*, and Xiaoyan Tang1,2*   

  1. 1Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China
    2Shenzhen Institute of Molecular Crop Design, Shenzhen 518107, China
    3School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China

    These authors contribute equally to this work
    *Correspondences: Jianxin Wu (wjxin@m.scnu.edu.cn); Xiaoyan Tang (txy@frontier-ag.com, Dr. Tang is fully responsible for the distribution of all materials associated with this article.)
  • Received:2019-10-29 Accepted:2019-12-10 Online:2019-12-13 Published:2020-08-01


Pollen grains are covered by exine that protects the pollen from stress and facilitates pollination. Here we isolated a male sterile mutant s13283 in rice exhibiting aborted pollen with abnormal exine and defective aperture. The mutant gene encodes a novel plasma membrane‐localized legume‐lectin receptor kinase that we named OsLecRK‐S.7. OsLecRK‐S.7 was expressed at different levels in all tested tissues and throughout anther development. In vitro kinase assay showed OsLecRK‐S.7 capable of autophosporylation. Mutation in s13283 (E560K) and mutation of the conserved ATP binding site (K418E) both knocked out the kinase activity. Mass spectrometry showed Thr376, Ser378, Thr386, Thr403, and Thr657 to be the autophosphorylation sites. Mutation of individual autophosphorylation site affected the in vitro kinase activity to different degrees, but did not abolish the gene function in fertility complementation. oslecrk‐s.7 mutant plant overexpressing OsLecRK‐S.7 recovered male fertility but showed severe growth retardation with reduced number of tillers, and these phenotypes were abolished by E560K or K418E mutation. The results indicated that OsLecRK‐S.7 was a key regulator of pollen development.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q