J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (12): 1910-1925.DOI: 10.1111/jipb.12984

Special Issue: Nutrition and stress

• Plant-abiotic Interactions • Previous Articles     Next Articles

AtHAP5A regulates iron translocation in iron‐deficient Arabidopsis thaliana

Xiao Fang Zhu1, Qi Wu1,2, Yu Ting Meng1,2, Ye Tao1,2 and Ren Fang Shen1,2*   

  1. 1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China
    2University of Chinese Academy of Sciences, Beijing, 100049, China

    Email: Ren Fang Shen (rfshen@issas.ac.cn)
  • Received:2020-05-15 Accepted:2020-06-16 Online:2020-06-17 Published:2020-12-01


Iron (Fe) deficient plants employ multiple strategies to increase root uptake and root‐to‐shoot translocation of Fe. The identification of genes that are responsible for these processes, and a comprehensive understanding of the regulatory effects of transcriptional networks on their expression, including transcription factors (TFs), is underway in Arabidopsis thaliana. Here, we show that a Histone‐ or heme‐associated proteins (HAP) transcription factor (TF), HAP5A, is necessary for the response to Fe deficiency in Arabidopsis. Its expression was induced under Fe deficiency, and the lack of HAP5A significantly decreased Fe translocation from the root to the shoot, resulting in substantial chlorosis of the newly expanded leaves, compared with the wild‐type (WT, Col‐0). Further analysis found that the expression of a gene encoding nicotianamine (NA) synthase (NAS1) was dramatically decreased in the hap5a mutant, regardless of the Fe status. Yeast‐one‐hybrid and ChIP analyses suggested that HAP5A directly binds to the promoter region of NAS1. Moreover, overexpression of NAS1 could rescue the chlorosis phenotype of hap5a in Fe deficient conditions. In summary, a novel pathway was elucidated, showing that NAS1‐dependent translocation of Fe from the root to the shoot is controlled by HAP5A in Fe‐deficient Arabidopsis thaliana.

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