J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (1): 203-222.DOI: 10.1111/jipb.13438

• Molecular Physiology • Previous Articles     Next Articles

AtMCM10 promotes DNA replication-coupled nucleosome assembly in Arabidopsis

Xinjie Zhao1, Jingyi Wang2, Dan Jin1, Jinkui Cheng1, Hui Chen1, Zhen Li1, Yu Wang1, Huiqiang Lou3, Jian-Kang Zhu4, Xuan Du5,6*, Zhizhong Gong1,7*   

  1. 1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences China Agricultural University Beijing 100193 China;
    2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences The Chinese Academy of Agricultural Sciences Beijing 100081 China;
    3. State Key Laboratory of Agro-Biotechnology, College of Biological Sciences China Agricultural University Beijing 100193 China;
    4. Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Institute of Plant and Food Science, School of Life Science, Department of Biology Southern University of Science and Technology Shenzhen 518055 China;
    5. Department of Biochemistry and Molecular Biology, International Cancer Center Shenzhen University Medical School Shenzhen 518060 China;
    6. Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering Shenzhen University Medical School Shenzhen 518060 China;
    7. School of Life Science, Institute of Life Science and Green Development Hebei University Baoding 071002 China
    *Correspondences: Xuan Du (duxuan@szu.edu.cn); Zhizhong Gong (gongzz@cau.edu.cn, Dr. Gong is fully responsible for the distributions of all materials associated with this article)
  • Received:2022-12-07 Accepted:2022-12-19 Online:2022-12-21 Published:2023-01-01

Abstract: Minichromosome Maintenance protein 10 (MCM10) is essential for DNA replication initiation and DNA elongation in yeasts and animals. Although the functions of MCM10 in DNA replication and repair have been well documented, the detailed mechanisms for MCM10 in these processes are not well known. Here, we identified AtMCM10 gene through a forward genetic screening for releasing a silenced marker gene. Although plant MCM10 possesses a similar crystal structure as animal MCM10, AtMCM10 is not essential for plant growth or development in Arabidopsis. AtMCM10 can directly bind to histone H3-H4 and promotes nucleosome assembly in vitro. The nucleosome density is decreased in Atmcm10, and most of the nucleosome density decreased regions in Atmcm10 are also regulated by newly synthesized histone chaperone Chromatin Assembly Factor-1 (CAF-1). Loss of both AtMCM10 and CAF-1 is embryo lethal, indicating that AtMCM10 and CAF-1 are indispensable for replication-coupled nucleosome assembly. AtMCM10 interacts with both new and parental histones. Atmcm10 mutants have lower H3.1 abundance and reduced H3K27me1/3 levels with releasing some silenced transposons. We propose that AtMCM10 deposits new and parental histones during nucleosome assembly, maintaining proper epigenetic modifications and genome stability during DNA replication.

Key words: AtMCM10, H3K27me1, nucleosomes assembly

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q