J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (7): 1687-1702.DOI: 10.1111/jipb.13477

• Cell and Developmental Biology • Previous Articles     Next Articles

Young Leaf White Stripe encodes a P-type PPR protein required for chloroplast development

Jie Lan1†, Qibing Lin2†, Chunlei Zhou1†, Xi Liu1, Rong Miao1, Tengfei Ma1, Yaping Chen1, Changling Mou1, Ruonan Jing1, Miao Feng2, Thanhliem Nguyen1, Yulong Ren2, Zhijun Cheng2, Xin Zhang2, Shijia Liu1, Ling Jiang1* and Jianmin Wan1,2*   

  1. 1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China;
    2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    These authors contributed equally to this work.
    *Correspondences: Jianmin Wan (wanjm@njau.edu.cn; Dr. Wan is responsible for the distribution of the materials associated with this article); and Ling Jiang (jiangling@njau.edu.cn)
  • Received:2022-12-10 Accepted:2023-03-07 Online:2023-03-10 Published:2023-07-01

Abstract: Pentatricopeptide repeat (PPR) proteins function in post-transcriptional regulation of organellar gene expression. Although several PPR proteins are known to function in chloroplast development in rice (Oryza sativa), the detailed molecular functions of many PPR proteins remain unclear. Here, we characterized a rice young leaf white stripe (ylws) mutant, which has defective chloroplast development during early seedling growth. Map-based cloning revealed that YLWS encodes a novel P-type chloroplast-targeted PPR protein with 11 PPR motifs. Further expression analyses showed that many nuclear- and plastid-encoded genes in the ylws mutant were significantly changed at the RNA and protein levels. The ylws mutant was impaired in chloroplast ribosome biogenesis and chloroplast development under low-temperature conditions. The ylws mutation causes defects in the splicing of atpF, ndhA, rpl2, and rps12, and editing of ndhA, ndhB, and rps14 transcripts. YLWS directly binds to specific sites in the atpF, ndhA, and rpl2 pre-mRNAs. Our results suggest that YLWS participates in chloroplast RNA group II intron splicing and plays an important role in chloroplast development during early leaf development.

Key words: chloroplast development, PPR protein, ribosome biogenesis, RNA splicing

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