J Integr Plant Biol ›› 2014, Vol. 56 ›› Issue (4): 400-410.DOI: 10.1111/jipb.12138

Special Issue: Rice Genomics and Agriculture

• Molecular Physiology • Previous Articles     Next Articles

The rice OsV4 encoding a novel pentatricopeptide repeat protein is required for chloroplast development during the early leaf stage under cold stress

Xiaodi Gong1†, Qianqian Su1†, Dongzhi Lin1†*, Quan Jiang1, Jianlong Xu2, Jianhui Zhang1, Sheng Teng3 and Yanjun Dong1*   

  1. 1Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, China
    2Institute of Crop Sciences, the Chinese Academy of Agricultural Sciences, Beijing, China
    3Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, the Chinese Academy of Sciences, Shanghai, China
  • Received:2013-10-24 Accepted:2013-11-27 Published:2013-11-30
  • About author:These authors contributed equally to this work.
    **Corresponding E-mail: dzlin@shnu.edu.cn; dong@shnu.edu.cn

Abstract:

Pentatricopeptide repeat (PPR) proteins, characterized by tandem arrays of a 35 amino acid motif, have been suggested to play central and broad roles in modulating the expression of organelle genes in plants. However, the molecular mechanisms of most rice PPR genes remains unclear. In this paper, we isolated and characterized a temperature-conditional virescent mutant, OsV4, in rice (Oryza sativa cultivar Jiahua1 (WT, japonica rice variety)). The mutant displays albino phenotype and abnormal chloroplasts at the three leaf stage, which gradually turns green after the four leaf stage at a low temperature (20 °C). But the mutant always develops green leaves and well-developed chloroplasts at a high temperature (32 °C). Genetic and molecular analyses uncovered that OsV4 encodes a novel chloroplast-targeted PPR protein including four PPR motifs. Further investigations show that the mutant phenotype is associated with changes in chlorophyll content and chloroplast development. The OsV4 transcripts only accumulate to high levels in young leaves, indicating that its expression is tissue-specific. In addition, transcript levels of some ribosomal components and plastid-encoded polymerase-dependent genes are dramatically reduced in the albino mutants grown at 20 °C. These findings suggest that OsV4 plays an important role during early chloroplast development under cold stress in rice.

Gong X, Su Q, Lin D, Jiang Q, Xu J, Zhang J, Teng S, Dong Y (2014) The rice OsV4 encoding a novel pentatricopeptide repeat protein is required for chloroplast development during the early leaf stage under cold stress. J Integr Plant Biol 56: 400–410. doi: 10.1111/jipb.12138

Key words: Chloroplast development, pentatricopeptide repeat, plastid-encoded polymerase, rice, virescent

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