J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (7): 1826-1840.DOI: 10.1111/jipb.13482

• Plant Biotic Interactions • Previous Articles    

The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the geminiviral infection in Arabidopsis

Tabata Rosas‐Diaz1,2, Pepe Cana‐Quijada1, Mengshi Wu2, Du Hui3, Gemma Fernandez‐Barbero4, Alberto P. Macho2, Roberto Solano4, Araceli G. Castillo1, Xiao‐Wei Wang3, Rosa Lozano‐Duran2,5* and Eduardo R. Bejarano1*   

  1. 1. Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM‐UMA‐CSIC), Universidad de Málaga, Málaga, Spain;
    2. Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China;
    3. Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China;
    4. Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología‐Consejo Superior de Investigaciones Científicas, Madrid 28049, Spain;
    5. Department of Plant Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Tübingen D‐72076, Germany
    *Correspondences: Rosa Lozano‐Duran (rosa.lozano-duran@zmbp.uni-tuebingen.de); Eduardo R. Bejarano (edu_rodri@uma.es, Dr. Bejarano is responsible for the distribution of the materials associated with this article)
  • Received:2022-09-06 Accepted:2023-03-17 Online:2023-03-22 Published:2023-07-01

Abstract: Jasmonates (JAs) are phytohormones that finely regulate critical biological processes, including plant development and defense. JASMONATE ZIM-DOMAIN (JAZ) proteins are crucial transcriptional regulators that keep JA-responsive genes in a repressed state. In the presence of JA-Ile, JAZ repressors are ubiquitinated and targeted for degradation by the ubiquitin/proteasome system, allowing the activation of downstream transcription factors and, consequently, the induction of JA-responsive genes. A growing body of evidence has shown that JA signaling is crucial in defending against plant viruses and their insect vectors. Here, we describe the interaction of C2 proteins from two tomato-infecting geminiviruses from the genus Begomovirus, tomato yellow leaf curl virus (TYLCV) and tomato yellow curl Sardinia virus (TYLCSaV), with the transcriptional repressor JAZ8 from Arabidopsis thaliana and its closest orthologue in tomato, SlJAZ9. Both JAZ and C2 proteins colocalize in the nucleus, forming discrete nuclear speckles. Overexpression of JAZ8 did not lead to altered responses to TYLCV infection in Arabidopsis; however, knock-down of JAZ8 favors geminiviral infection. Low levels of JAZ8 likely affect the viral infection specifically, since JAZ8-silenced plants neither display obvious developmental phenotypes nor present differences in their interaction with the viral insect vector. In summary, our results show that the geminivirus-encoded C2 interacts with JAZ8 in the nucleus, and suggest that this plant protein exerts an anti-geminiviral effect.

Key words: C2 protein, geminivirus, jasmonates, JAZ8 transcriptional repressor, TYLCV

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