J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (8): 2001-2017.DOI: 10.1111/jipb.13490

• Plant Reproductive Biologys • Previous Articles    

Arabidopsis pollen-specific glycerophosphodiester phosphodiesterase-like genes are essential for pollen tube tip growth

Chong Wang1, Hao Cheng1, Wenjing Xu1, Jingshi Xue2, Xinguo Hua1, Guimin Tong1, Xujun Ma1, Chuanping Yang1, Xingguo Lan1,3*, Shi‐Yi Shen2, Zhongnan Yang2, Jirong Huang2 and Yuxiang Cheng1*   

  1. 1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China;
    2. Shanghai Key Laboratory of Plant Molecular Sciences, Shanghai Normal University, Shanghai 200234, China;
    3. College of Life Sciences, Northeast Forestry University, Harbin 150040, China
    *Correspondences: Xingguo Lan (lanxingguo@nefu.edu.cn); Yuxiang Cheng (chengyuxiang@nefu.edu.cn, Dr. Yuxiang Cheng is fully responsible for the distribution of the materials associated with this article)
  • Received:2022-09-24 Accepted:2023-03-31 Online:2023-04-04 Published:2023-08-01

Abstract: In angiosperms, pollen tube growth is critical for double fertilization and seed formation. Many of the factors involved in pollen tube tip growth are unknown. Here, we report the roles of pollen-specific GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE-LIKE (GDPD-LIKE) genes in pollen tube tip growth. Arabidopsis thaliana GDPD-LIKE6 (AtGDPDL6) and AtGDPDL7 were specifically expressed in mature pollen grains and pollen tubes and green fluorescent protein (GFP)-AtGDPDL6 and GFP-AtGDPDL7 fusion proteins were enriched at the plasma membrane at the apex of forming pollen tubes. Atgdpdl6 Atgdpdl7 double mutants displayed severe sterility that was rescued by genetic complementation with AtGDPDL6 or AtGDPDL7. This sterility was associated with defective male gametophytic transmission. Atgdpdl6 Atgdpdl7 pollen tubes burst immediately after initiation of pollen germination in vitro and in vivo, consistent with the thin and fragile walls in their tips. Cellulose deposition was greatly reduced along the mutant pollen tube tip walls, and the localization of pollen-specific CELLULOSE SYNTHASE-LIKE D1 (CSLD1) and CSLD4 was impaired to the apex of mutant pollen tubes. A rice pollen-specific GDPD-LIKE protein also contributed to pollen tube tip growth, suggesting that members of this family have conserved functions in angiosperms. Thus, pollen-specific GDPD-LIKEs mediate pollen tube tip growth, possibly by modulating cellulose deposition in pollen tube walls.

Key words: Arabidopsis thaliana, cell wall integrity, cellulose deposition, male sterility, pollen-specific GDPD-LIKE, pollen tube tip growth

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