J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (9): 2071-2085.DOI: 10.1111/jipb.13534

• Cell and Developmental Biology • Previous Articles     Next Articles

ERF5 enhances protocorm-like body regeneration via enhancement of STM expression in Dendrobium orchid

Danqi Zeng1,2,3, Can Si1,3, Mingze Zhang1, Jun Duan1,2,3,4* and Chunmei He1,2,3*   

  1. 1. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;
    2. University of the Chinese Academy of Sciences, Beijing 100049, China;
    3. South China National Botanical Garden, Guangzhou 510650, China;
    4. Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou 510650, China
    *Correspondences: Chunmei He (hechunmei2012@scbg.ac.cn, Dr. He is fully responsible for the distributions of all materials associated with this article); Jun Duan (duanj@scib.ac.cn)
  • Received:2023-01-04 Accepted:2023-05-19 Online:2023-05-22 Published:2023-09-01

Abstract: Orchid plants develop protocorms upon germination and produce protocorm-like structures called protocorm-like bodies (PLBs) from protocorms and somatic cells via tissue culture. Protocorm-like bodies have broad technical application potential in the orchid industry and their regeneration is a distinct developmental process in the plant kingdom. However, little is known about this unparalleled developmental program. In this study, we identified a PLB-abundant gene, ethylene response factor (ERF), and a transcription factor named DoERF5, and determined its important role in PLB regeneration in Dendrobium orchid. Overexpression of DoERF5 in Dendrobium greatly enhanced the PLB regeneration from PLB and stem explants, and upregulated the expression of WOUND-INDUCED DEDIFFERENTIATION (DoWIND) homologs and SHOOT MERISTEMLESS (DoSTM), as well as the genes involved in cytokinin biosynthesis (DoIPT) and the cytokinin response factors (DoARRs). However, silencing DoERF5 reduced the regeneration rate of PLBs, and downregulated the expression of DoWIND homologs, DoSTM and DoARRs. We demonstrated that DoERF5 is directly bound to the DoSTM promoter and regulates its expression. In addition, overexpression of DoSTM in Dendrobium orchid resulted in favorable regeneration of PLBs. Our results clarify that DoERF5 regulates the regeneration of PLB by enhancing DoSTM expression. Our findings provide new insights into how DoERF5 mediates PLB regeneration and offers technical potential in improving clonal propagation, preservation, and the bioengineering of orchids.

Key words: EMSA, ERF transcription factor, orchid, protocorm-like body, STM

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