The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution

doi:10.1111/jipb.13744

J Integr Plant Biol. ›› 2024, Vol. 66 ›› Issue (9): 2058-2075.DOI: 10.1111/jipb.13744

• Plant Biotic Interactions • Previous Articles

The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution

Yuan‐Bao Li^1,2, Chengyu Liu^1,3, Ningning Shen^1,2, Shuai Zhu^1,2, Xianya Deng^1,4, Zixuan Liu^1,2, Li‐Bo Han^1,2* and Dingzhong Tang^1,2*

1. State Key Laboratory of Ecological Control of Fujian‐Taiwan Crop Pests, Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2. College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
4. School of Future Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
^*Correspondences: Li‐Bo Han (Hanlibo@im.ac.cn); Dingzhong Tang (dztang@fafu.edu.cn, Dr. Tang is fully responsible for the distributions of the material associated with this article)

Received:2024-03-17 Accepted:2024-06-20 Online:2024-07-23 Published:2024-09-01
Supported by:
This work was funded by the National Key Research and Development Program of China (2022YFF1001500); the National Natural Science Foundation of China (grant numbers 31970284, 31900385); the Natural Science Foundation of Fujian Province, China (2023J01483, 2022J01616); the Distinguished Young Scientists Fund of Fujian Agriculture and Forestry University of China (xjq202121); and the Fujian Provincial Science and Technology Key Project (2022NZ030014).

Abstract

Abstract: Magnaporthe oryzae (M. oryzae) is a devastating hemibiotrophic pathogen. Its biotrophic invasive hyphae (IH) are enclosed in the extrainvasive hyphal membrane produced by plant cells, thus generating a front line of the battlefield between the pathogen and the host plants. In plants, defense-related complexes such as proteins, callose-rich materials and vesicles, are directionally secreted to this interface to confer defense responses, but the underlying molecular mechanism is poorly understood. In this study, we found that a Myosin gene, Myosin A1 (OsMYA1), contributed to rice defense. The OsMYA1 knockout mutant exhibited decreased resistance to M. oryzae infection. OsMYA1 localizes to the actin cytoskeleton and surrounds the IH of M. oryzae. OsMYA1 interacts with an exocyst subunit, OsExo70H1, and regulates its accumulation at the plasma membrane (PM) and pathogen–plant interface. Furthermore, OsExo70H1 interacted with the rice syntaxin of the plants121 protein (OsSyp121), and the distribution of OsSyp121 to the PM or the pathogen–plant interface was disrupted in both the OsMYA1 and OsExo70H1 mutants. Overall, these results not only reveal a new function of OsMYA1 in rice blast resistance, but also uncover a molecular mechanism by which plants regulate defense against M. oryzae by OsMYA1-initiated vesicle secretory pathway, which originates from the actin cytoskeleton to the PM.

Key words: cytoskeleton, plant defense mechanism, rice blast, vesicle delivery

Yuan‐Bao Li, Chengyu Liu, Ningning Shen, Shuai Zhu, Xianya Deng, Zixuan Liu, Li‐Bo Han and Dingzhong Tang. The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution[J]. J Integr Plant Biol., 2024, 66(9): 2058-2075.

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The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution

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