Integrative Biology Journals
JIPB

J Integr Plant Biol.

• Research Article • Previous Articles    

OsCPN10a, cooperating with OsCPN20 and OsHSP60‐3B negatively regulate ABA signaling and enhance seed storability in rice

Sufeng Liao1,2,3,4†, Yidong Wei1,3,4†, Yongsheng Zhu1,3,4, Kunyang Li1,3,4, Ting Chen1,2,3,4, Shuai Zhao5, Fangxi Wu1,2,3,4, Jinlan Wang2,3,4, Pengbo Yu2, Hongguang Xie1,2,3,4, Liping Chen2,3,4, Qiuhua Cai2,3,4, Huaan Xie1,2,3,4 and Jianfu Zhang1,2,3,4*   

  1. 1. Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China
    2. Cross‐straits Agricultural Technology Cooperation Center, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    3. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Affairs, Fuzhou 350003, China
    4. Incubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences and Technology, Fujian Engineering Laboratory of Crop Molecular Breeding, Fujian Key Laboratory of Rice Molecular Breeding, National Rice Improvement Center of China, Fuzhou 350003, China
    5. Key Laboratory for Basic and Applied Research of Bayu Prescriptions and Herbs, College of Chinese Materia Medica, Chongqing University of Chinese Medicine, Chongqing 361000, China
    †These authors contributed equally to this article.
    *Correspondence: Jianfu Zhang (jianfzhang@163.com)
  • Received:2026-01-08 Accepted:2026-02-20 Online:2026-03-16
  • Supported by:
    This work was supported by the National Rice Industry Technology System of Modern Agriculture for China (Grant no. CARS‐01‐08), Design and Breeding of New High‐yield Hybrid Indica Rice Varieties in South China Rice‐Growing Areas, Science and Technology Innovation 2030—Major Projects, China (Grant no. 2023ZD040220306), Creation and Application of New Germplasm Sources of high‐ quality and Delicious Hybrid Rice in Fujian, China (Grant no. NK2022050604‐3), the Key Science and Technology Projects of Fujian Academy of Agricultural Sciences (Grant No. KJZD202401), and the Key Program of Science and Technology in Fujian Province, China (Grant no. 2024NZ029027).

HTML

PDF

Abstract: Seed storability is crucial for maintaining vigor at the sowing stage and ensuring agricultural sustainability. Although molecular chaperones are well characterized in protein homeostasis, their roles in regulating ABA receptor stability and starch integrity during seed aging remain unclear. Here, we demonstrate that OsCPN10a enhances storability by maintaining ABA homeostasis, suppressing ROS accumulation, and preserving starch granule structure. Genetic and biochemical analyses reveal that OsCPN10a functions upstream of the OsCPN20–OsHSP60‐3B cascade, directly interacts with both partners to promote complex assembly, and negatively regulates ABA signaling. The resulting trimeric complex interacts with OsPYL10, facilitating receptor dissociation and high‐affinity association with the PP2C phosphatase OsABIL1 to attenuate signaling. This study reveals a non‐canonical function of molecular chaperones in directly modulating hormone receptor signaling, establishing a mechanistic link between protein homeostasis and ABA sensitivity in seeds.

Key words: ABA signaling, molecular chaperone, Oryza sativa, OsCPN10a, ROS homeostasis, seed storability, starch integrity

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2026 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号：京ICP备16067583号-22