Efficient genome editing in tomato using Cas12i3 variants
Siqun Wu1†, Lei Zhu1, 2†, Meili Zhou2, Bo Yu3, Jinkai Li4, Xiaomu Niu2, Pengcheng Wang1, Jian-Kang Zhu1, 5, 6* and Zhaobo Lang1*
1. Institute of Advanced Biotechnology, Institute of Homeostatic Medicine and School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China; 2. Bellagen Biotechnology Co., Ltd, Jinan 250000, China; 3. Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 4. BGI Research, Shenzhen 518083, China; 5. Key Laboratory of Gene Editing Technologies, Ministry of Agricultural and Rural Affairs/National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572024, China; 6. School of Medicine, Macau University of Science and Technology, Macau SAR 999078, China †These authors contributed equally to this work. *Correspondences: Zhaobo Lang (langzb@sustech.edu.cn, Dr. Lang is fully responsible for the distribution of all materials associated with this article); Jian-Kang Zhu (zhujk@sustech.edu.cn)
This work was supported by the Agriculture Science and Technology Major Project, and National Key R&D Program of China (Grant numbers: 2021YFA1300404), the National Natural Science Foundation of China (Grant numbers: 31900394, 31671735, and 31970516), the Key R&D Program of Shandong Province, China (Competitive Innovation Platform) (2023CXPT095), the Shenzhen Science and Technology Program (Grant numbers: JCYJ20241202125311016 KQTD20240729102038044, and KJZD20240903102703005), and Shandong Shunfeng Biotechnology Co., Ltd., Jinan, China.
