J Integr Plant Biol.
• Brief Communication •
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T2T genome assembly and CRISPR/Cas9 editing of Medicago lupulina
Qing Yuan1, 2†, Cheng Li1†, Guangyan Wang1†, Wenfei Dai1, 3, Eerdungaridi4, Di Wu4, Qingdun Fan1, Yongping Yang1, Yuanwen Duan1, Xudong Sun1
- 1. Yunnan Key Laboratory of Crop Wild Relatives Omics, The Germplasm Bank of Wild Species, Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
2. School of Ecology and Environmental Science, Yunnan University, Kunming 650500, China;
3. National Technology Innovation Center for Prataculture, Hohhot 010000, China
†These authors contributed equally to this work.
*Correspondences: Yuanwen Duan (duanyw@mail.kib.ac.cn, Dr. Duan is fully responsible for the distribution of all materials associated with this article); Xudong Sun (sunxudong@mail.kib.ac.cn)
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Received:2026-06-02
Revised:2026-09-07
Accepted:2026-09-07
Online:2026-09-19
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Supported by:We gratefully thank Dr. Caspar C. C. Chater (Royal Botanic Gardens, UK) for revising the manuscript for writing style, English language, and syntax. This work was financially supported by the National Technology Innovation Center for Prataculture Special fund for innovation platform construction (CCPTZX2024GJ11), the Major Science and Technology Project of Qinghai Province (2025-NK-A1-01), the Major Program for Basic Research Project of Yunnan Province (202401BC070001), the Major Program of Yunnan Province (202602AE090029), and the National Natural Science Foundation of China (32371586, 32370405, and 32500309).