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Engineering AP/Exo-Cas12 as an efficient toolbox for rice genome editing via integrated repair and exonuclease modules

Nan Chai1†, Ruixiang Zhang1†, Guangzhou Li1†, Liying Su1, Jun Wen1, Shirui Huo1, Xudong Yuan1, Ruyue Chang1, Xinglei Zheng1, Zhiye Zheng1, Taoli Liu1, Dongchang Zeng2, Qiupeng Lin1, Yao-Guang Liu1, Xianrong Xie1*, Jiantao Tan3*, Qinlong Zhu1*   

  1. 1. Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops, College of Agriculture, South China Agricultural University, Guangzhou 510642, China;
    2. College of Life Sciences, Guangxi Normal University, Guilin 541006, China;
    3. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
    †These authors contributed equally to this work.
    *Correspondences: Qinlong Zhu (zhuql@scau.edu.cn, Dr. Zhu is fully responsible for the distribution of all materials associated with this article); Jiantao Tan (tanjiantao@gdaas.cn); Xianrong Xie (xiexianrong@scau.edu.cn)
  • Received:2026-05-15 Revised:2026-10-17 Accepted:2026-10-17 Online:2026-11-03
  • Supported by:
    This work was supported by the Agriculture Science and Technology Major Project, the Key R&D Program of Guangdong Province (2025B0202050004), the Guangxi Science and Technology Major Project (GKAA24206023), and the Guangdong Basic Research Center of Excellence for Precise Breeding of Future Crops Major Project (FCBRCE-202612).
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