Abstract:

Using a computer software `uniform design, regression analysis and optimization system' (UROS), the effects of Armillaria mellea Karst elicitor, with respect to its concentration, time of supplement and harvest time, on the accumulation of tanshinones in the crown gall cultures of Salvia miltiorrhiza Bunge in 67-V liquid medium were studied. The result showed that the maximum tanshinone yield was 147 mg/L (P＜0.05）, 62 mg/L (P＜0.05）, 94 mg/L (P＜0.05） in the liquid medium and the cultures 29 day after addition of A. mellea (119 mL/L) at day 0, in the medium 26 day after the addition of 113 mL/L of A. mellea, 28 day after addition of 87 mL/L of A. mellea, respectively. The results confirmed that the computer software UROS was a good tool in the multi-factor study.

蜜环菌诱导子对丹参冠瘿组织积累丹参酮的影响
宋经元¹ 祁建军¹ 雷和田¹ 任春玲²  付洁¹ 张荫麟¹

（1. 中国医学科学院中国协和医科大学药用植物研究所，北京100094；2. 河北省科学院微生物研究所，保定071051）

摘要：以６７－Ｖ液体培养基为基本培养基，利用计算机软件“均匀设计、回归分析及优化系统”，研究了蜜环菌（Ａｒｍｉｌｌａｒｉａ ｍｅｌｌｅａ Ｋａｒｓｔ）诱导子浓度、诱导子的加入时间与处理的收获时间对丹参（Ｓａｌｖｉａ ｍｉｌｔｉｏｒｒｈｉｚａ Ｂｕｎｇｅ）冠瘿组织积累丹参酮的影响。结果表明，蜜环菌诱导子浓度为１１９ｍＬ／Ｌ、第０天加入诱导子、第２９天收获培养物与培养液时可获得最高的丹参酮生产量（１４７ｍｇ/L,P<0.05），蜜环菌诱导子浓度为113mL/L第0 天加入诱导子、第26天收
获培养液时可获得最高的丹参酮生产量（62mg/L,P<0.05），蜜环菌诱导子浓度87mL/L,、第0天加入诱导子、第28天收获培养物时可获得最高的丹参酮生产量（94mg/L,P<0.05）。结果证实，计算机软件“均匀设计、回归分析及优化系统”对于观察值受多因素影响的研究非常有效和方便。
关键词： 丹参；冠瘿组织；蜜环菌；诱导子；丹参酮；均匀设计、回归分析及优化系统

Key words: Salvia miltiorrhiza, crown gall cultures, Armillaria mellea, elicitor, tanshinones, uniform design, regression analysis and optimization system (UROS)