J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (7): 689-692.

• Research Articles • Previous Articles     Next Articles

The Relation Between C4 Pathway Enzymes and PSII Photochemical Function in Soybean

LI Wei-Hua, LU Qing-Tao, HAO Nai-Bin, GE Qiao-Ying, ZHANG Qi-De, JIANG Gao-Ming, DU Wei-Guang, KUANG Ting-Yun   


Four C4 pathway enzymes of “Heinong 41" leaves of soybeans (Glycine max (L) Merr.) were assayed in five developmental stages. Pn (the net photosynthetic rate) and the chlorophyll fluorescence parameters (Fv/Fo, qP, qN and ΦPSⅡ) were also measured. The results indicated that the activities of C4 enzymes, Pn, the chlorophyll fluorescence parameters and the ratio of PEPCase (PEP carboxylase)/RuBPCase (ribulose-1,5-biphosphate) shared the same changing trait during the whole developmental stages of “Heinong 41". Correlation analysis showed that the activities of C4 enzymes, Pn, the chlorophyll fluorescence parameters were positively correlated with the ratio of PEPCase/RuBPCase. All these suggested that the degree of C4 pathway expression in “Heinong 41" leaves were positively correlated with its Pn; the photochemical function of PSⅡ was adjusted to meet the specific energy requirements for the operation of C4 pathway with the improvement of C4 expression.

李卫华1 卢庆陶1 郝乃斌1* 戈巧英1 张其德1 蒋高明1 杜维广2  匡廷云1

(1. 中国科学院植物研究所,北京100093;2. 黑龙江省农业科学院大豆研究所,哈尔滨150086)

摘要:测定了不同发育时期大豆(Glycine max(L)Merr.)“黑农41”叶片的4种C4酶(PEPCase(磷酸烯醇式丙酮酸羧化酶)、NADP-MDH(NADP苹果酸脱氢酶)、NADP-ME(NADP苹果酸酶)和PPDK(丙酮酸磷酸二激酶))活性、荧光动力学数值(Fv/Fo(PSⅡ活性)、qP(光化学淬灭)、qN(非光化学淬灭、ΦPSⅡ(有效PSⅡ光化学效率))和光合速率。结果表明在“黑农41”的整个生育期C4途径的表达具有一定的规律:即与净光合速率密切相关;而且随着/.途径在大豆体内表达程度的提高,大豆叶片PSII对原初光能的吸收、传递和转化能力都随之提高,以满足C4途径运行对能量的更多需求;同时C4途径的存在也提高了“黑农41”叶片对光能的吸收、传递和转化。
关键词: 大豆;C4途径酶;荧光动力学;光系统!;相关

Key words: soybean, C4 pathway enzyme, chlorophyll fluorescence, PSⅡ, correlation

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