J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (7): 684-688.

• Research Articles • Previous Articles     Next Articles

Isolation of Chlorophyll Protein Complexes from the Thylakoid Membrane in Nostoc flagelliforme and Their Spectroscopic Characterization

WANG Mei, XU Yi-Nong, JIANG Gui-Zhen, LI Liang-Bi, KUANG Ting-Yun   


Nostoc flagelliforme Born. et Flah is highly adapted to drought stress, cold and light stresses, and suitable for growing in the unfavorable areas. This paper presents the results of the analysis of the membrane (mainly thylakoid membrane) lipids from N. flagelliforme in order to investigate the relationship between membrane lipid composition and stress resistance to this cyanobacteria. The membrane lipids are composed of monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG), sulfoquinovosyl diacylglycerol (SQDG) and phosphatidylglycerol (PG). The major fatty acids in these lipids are palmitic (16∶0), palmitoleic (16∶1), stearic (18∶0), oleic (18∶1), linoleic (18∶2) and linolenic (18∶3) acids. In N. flagelliforme, polyunsaturated fatty acids account for 73% of the total fatty acids, much higher than that of the other cyanobacteria reported so far. Among which 16∶1 and 18∶3 are as high as 28.9% and 34.3% respectively. The high resistance of N. flagelliforme to abnormal conditions may be associated with the extent of unsaturation of fatty acids. In addition, the wild N. flagelliforme treated with water for 30 min and cultured for 24 h and the lipid and fatty acid composition were found to be not affected by water-absorption.

王梅 钟泽璞* 王可玢 李良璧 匡廷云

摘要:采用改进的Allen’s的绿胶系统 ,首次对陆生蓝藻发菜 (Nostocflagelliforme Born .etFlah .)类囊体膜色素蛋白复合物进行了分离 ,共分离出了 11条绿色的色素蛋白复合物条带 ,两条浅黄色的条带。其中 7条绿色条带属于PSⅠ组分 ,4条绿色条带属于PSⅡ组分 ,1条浅黄色条带经光谱分析初步认定为类胡萝卜素蛋白复合物 ,而另一条浅黄色条带为游离色素。

关键词: 发菜;类囊体膜;叶绿素蛋白复合物;光系统!;光系统";类胡萝卜素束缚蛋白

Key words: Nostoc flagelliforme, membrane lipids, fatty acids, cold resistance

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q