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J Integr Plant Biol, 2000, 42 (9): 952-956, Research Article
Creation and Cytological, Biochemical, Molecular Identification of Alien Disomic Substitution Lines with BYDV-resistance from Triticum aestivum-Agropyron intermedium Hybrids
TANG Shun-Xue
doi:
Abstract

By the method of combined anther culture and conventional selection, six new BYI)V (barley yellow dwarf vims)-resistant wheat germplasms (2n = 42) were obtained from the crosses between common wheat cv. 77-5433 and Zhong 5, a partial Triticum aestivum-Agrotryron intermedium amphiploid. The six germplasms were comprehensively identified by meiosis pairing analysis, testing cross analysis using monosomic analysis, C-banding, isoelectrofocusing (IEF) of isoenzyme and RAPD analyses. The results indicated that all the germplasms were alien substitution lines carrying resistant genes originated from Zhong 5.

抗大麦黄矮病毒小麦!中间偃麦草二体异代换系的选育和细胞、生化、分子生物学鉴定
唐顺学1 李义文1 梁辉1 曲乐庆1 白建荣1  贾双娥1 魏晓丽1 李振声1 贾旭1  B.FR5EBE2

(1. 中国科学院遗传研究所植物细胞与染色体工程国家重点实验室,北京100101;2. D.epartment  of Pathology,Kansas Unixer,Manhattan KS 66506-5501 USA

摘要:结合花药培养和常规选育 ,从 77- 433×中 5杂交组合后代中选育出 6个抗大麦黄矮病毒 (barleyyellowdwarfvirus ,BYDV)、染色体数目为 42的小麦 (TriticumaestivumL .)新种质 ,并用减数分裂配对分析、单体小麦测交法、基因组原位杂交、C分带、同工酶等电聚焦和RAPD对上述材料进行了综合鉴定 ;表明这些材料都是小麦_中间偃麦草(AgropyronintermediumGarten)二体异代换系 ,其抗病性来自其携带的一对中间偃麦草染色体

关键词: 中5;BYDV抗病种质;二体异代换系;细胞、生化、分子生物学鉴定

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