Abstract:

The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.)Kitag) were constructed by Southern blot analysis of Ase Ⅰ-，Dra Ⅰ-，Eco RⅠ-，Hin dⅢ-，NcoⅠ-，MobⅠ-，RsaⅠ-，PstⅠ-， TaqⅠ- digested genomic DNA probed with synthesized oligonucleotide,（CT）8，（GCTA）4，（GGAT） 4 or（GACA） 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ-or Rsa Ⅰ- digested genomic DNAs probed with GCTA）4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and（CT）8，（GCTA）4，（GGAT）4　or（GACA）4 as primer. Five size 2325，1455，876，774 and 299 bp fragments were amplified when (GGAT) 4 was used, indicating satisfied results were obtained.

用微卫星序列构建羊草遗传指纹图谱
刘杰 刘公社* 齐冬梅 李芳芳
（中国科学院植物研究所，北京100093）
关键词： 羊草；指纹图谱；SSR

Key words: Chinese wildrye, fingerprint, SSR (simple sequence repeats)