J Integr Plant Biol. ›› 2000, Vol. 42 ›› Issue (9): 985-987.

• Research Articles • Previous Articles     Next Articles

Construction of Genetic Fingerprints of Aneurolepidium chinensis Using Microsatellite Sequences

LIU Jie, LIU Gong-She , QI Dong-Mei, LI Fang-Fang   


The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.)Kitag) were constructed by Southern blot analysis of Ase Ⅰ-,Dra Ⅰ-,Eco RⅠ-,Hin dⅢ-,NcoⅠ-,MobⅠ-,RsaⅠ-,PstⅠ-, TaqⅠ- digested genomic DNA probed with synthesized oligonucleotide,(CT)8,(GCTA)4,(GGAT) 4 or(GACA) 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ-or Rsa Ⅰ- digested genomic DNAs probed with GCTA)4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and(CT)8,(GCTA)4,(GGAT)4 or(GACA)4 as primer. Five size 2325,1455,876,774 and 299 bp fragments were amplified when (GGAT) 4 was used, indicating satisfied results were obtained.

刘杰 刘公社* 齐冬梅 李芳芳
关键词: 羊草;指纹图谱;SSR

Key words: Chinese wildrye, fingerprint, SSR (simple sequence repeats)

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q