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J Integr Plant Biol, 2000, 42 (9): 985-987, Research Article
Construction of Genetic Fingerprints of Aneurolepidium chinensis Using Microsatellite Sequences
LIU Jie, LIU Gong-She , QI Dong-Mei, LI Fang-Fang
doi:
Abstract

The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.)Kitag) were constructed by Southern blot analysis of Ase Ⅰ-,Dra Ⅰ-,Eco RⅠ-,Hin dⅢ-,NcoⅠ-,MobⅠ-,RsaⅠ-,PstⅠ-, TaqⅠ- digested genomic DNA probed with synthesized oligonucleotide,(CT)8,(GCTA)4,(GGAT) 4 or(GACA) 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ-or Rsa Ⅰ- digested genomic DNAs probed with GCTA)4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and(CT)8,(GCTA)4,(GGAT)4 or(GACA)4 as primer. Five size 2325,1455,876,774 and 299 bp fragments were amplified when (GGAT) 4 was used, indicating satisfied results were obtained.

用微卫星序列构建羊草遗传指纹图谱
刘杰 刘公社* 齐冬梅 李芳芳
(中国科学院植物研究所,北京100093)
关键词: 羊草;指纹图谱;SSR

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