J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (1): 63-66.

• Research Articles • Previous Articles     Next Articles

Studies on Petunia hybrida Transformed with Flower-meristem-identity Gene AP1

AN Li-Xin, LIU Yi-Fei, CHEN Zhang-Liang, LI Yi   

Abstract:

Three deletion mutants of tobacco mosaic virus (TMV) 54-kD putative replicase gene (54K) were obtained by PCR, and cloned into plant expression vector p208, then transformed into Nicotiana tabacum L. cv. SR1 by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid-mediated transformation. All the transgenic plants with the N-terminal deletion mutant, the C-terminal deletion mutant and the only 261 nucleotides region from the central part of the 54K ORF showed significant resistance against TMV.

花分生组织决定基因AP1 转化矮牵牛的研究
安利忻1刘荣维2 陈章良1李毅1*

(1. 北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室,北京100871;2.  深圳农业科学中心花卉研究所,深圳518040)

利用RT-PCR方法从拟南芥 (Arabidopsisthaliana (L .)Heynh .)中克隆了花分生组织决定基因 (flower_meristem_identitygene)AP1,进行了全序列测定。测序结果显示 ,所得到的基因与发表的序列仅有一个碱基的差异 ,但并不影响蛋白质的一级结构。将AP1基因克隆入植物中间载体p2 0 8,通过根癌土壤杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化矮牵牛 (Petuniahybrida Vilm .)。对转基因植株进行了PCR和Southern检测 ,所得到的两个株系的转基因矮牵牛在R0 代即表现出提前且持续不断地开花的特性 ,与对照差异显著

关键词: 拟南芥;花分生组织决定基因;!./;矮牵牛

Key words: TMV, 54K mutants, replicase-mediated resistance

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