Abstract:

The blue-grained wheat substitution line (blue 58) originated from wild hybridization between Triticum aestivum L. and Agropyron elongatum (Host) Beauv= Elytrigia elongatum (Host) Nevski= Thinopyrum ponticum (Host) Barkworth and Dewey (2n=10x=70) was irradiated and four translocation lines were screened by fluorescence in situ hybridization from the offsprings. The results obtained include the following: (1) both the two translocation lines, 9906 and 9902, have 42 chromosomes. The length of the translocated blue-grained segment was approximately one-third of the short-arm and one-half of the long-arm of the translocated wheat chromosome in 9906 and 9902, respectively, and the blue-grained translocated segment in 9902 was located on D genome; (2) both 9915 and 9904 have 44 chromosomes. One pair of chromosomes was translocated and two chromosomes from Th. ponticum were added in 9903, while two pairs of chromosomes were translocated in 9904 by blue-grained wheat segment. The location and application of blue-grained wheat translocation lines were discussed.

蓝粒小麦易位系的荧光原位杂交鉴定
英加¹ 李滨¹ 穆素梅²  周汉平³ 刘建中¹ 李振声^1*

（1. 中国科学院遗传研究所植物细胞与染色体工程国家重点实验室，北京100101；
2. 中国科学院石家庄农业现代化研究所，石家庄050021；3. 中国科学院西北植物研究所，陕西杨712100）

摘要： 普通小麦（Triticum aestivum L.）和长穗偃麦草（Agropyronelongatum (Host) Beauv=Elytriga elongatum (Host) Nevski=Thinopyrum ponticum (Host) Barkworth and Dewey,2n=10x=70）杂交后选育出的蓝粒小麦异代换系（蓝58），经辐射诱导与选育，对其后代进行荧光原位杂交检测后获得:（1）9902，9906两个蓝粒易位系，体细胞染色体数均为2n=42，其中9906中被易位蓝粒片段的相对长度约占易位小麦染色体短臂的1/3，而9902中被易位蓝粒片段的相对长度约占易位小麦染色体长臂的1/2，并将9902的蓝粒易位片段定位在小麦D组染色体上；（2）9915易位附加和9904易位-易位附加，其体细胞染色体数均为44，其中9915的体细胞染色体只有一对发生了易位，另外附加了两条长穗偃麦草染色体；而9904有两对染色体发生了易位。并就易位系中控制蓝粒性状的长穗偃麦草染色体片段的定位和蓝粒小麦易位系的应用进行了讨论。

关键词： 蓝粒小麦；易位系；易位附加；易位-易位附加；荧光原位杂交