J Integr Plant Biol. ›› 2001, Vol. 43 ›› Issue (6): 592-597.

• Research Articles • Previous Articles     Next Articles

Aphid-resistant Transgenic Tobacco Plants Expressing Modified gna Gene

YUAN Zheng-Qiang, ZHAO Cun-You, ZHOU Yan, TIAN Ying-Chuan   

Abstract:

gene sequence coding for the precursor of Galanthus nivalis agglutinin (GNA) was modified by site-directed mutagenesis to change very low usage bias codons to higher usage bias ones for improvement of the gene expression in transgenic tobacco ( Nicotiana tabacum L.) plants. Results from Western blot analysis of some of the transgenic tobacco plants showed that the expression level of GNA in plants transformed with the modified gene GNA34m reached 0.25% of total soluble proteins, while that of the GNA34 gene transgenic plants was 0.17%. Since the GNA expression level increased, the aphid resistance of GNA34m transgenic plants were also enhanced significantly as judged by a 71.0% aphid population inhibition in insect bioassay of GNA34m transformed plants and 63.7% for the plants transformed with the natural GNA34 gene.

雪花莲凝集素基因(gna)的改造及其抗蚜性
袁正强 赵存友* 周岩 田颖川**

(中国科学院微生物研究所,北京100080)

摘要:用定点突变方法对编码雪花莲凝集素(Galanthus nivalis agglutinin, GNA)前体蛋白的DNA序列进行了改造和转基因烟草(Nicotiana tabacum L.)抗蚜性的研究.结果表明,将GNA编码序列中含有的稀有密码子改造后,GNA的表达水平从占总可溶性蛋白的0.17% 增加到0.25%,转基因烟草的抗蚜性也随之增强,从平均抑制桃蚜(Myzus persicae (Sulzer))虫口密度63.7%显著地提高到71.0%.

关键词: 雪花莲凝集素基因;定点突变;转基因烟草;抗蚜性

Key words: gna gene, site-directed mutagenesis, transgenic tobacco plants, aphid-resistance

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