Abstract:

Influence of the combination of nutrient feed with dissolved oxygen control on taxol production in suspension cultures of Taxus chinensis (Pilg.) Rehd. was investigated in bioreactors. Addition of feeding medium with 20 g/L sucrose on day 16 enhanced both the biomass and taxol production in 5 L bioreactors. Further investigation of the fed batch cultures in a 20 L bioreactor showed that cultivation under a low dissolved oxygen tension (DOT) of 20% for the entire culture resulted in the highest taxol content of 0.98 mg/g DW, while the taxol production was lower than that with 40% and 60% DOT. Moreover, taxol accumulation was remarkably improved by the cultivation of cells initially under DOT of 60% for 20 d followed by changing the DOT to 20% for another 12 d. By combining the use of these two strategies, a maximum taxol content of 18.7 mg/L was obtainedin a 20 L bioreactor.

补料培养与溶氧控制联合应用对红豆杉细胞培养的影响
罗杰 梅兴国
（华中科技大学生命科学与技术学院，武汉430074）

Key words: Taxus chinensis, taxol, nutrient feed, dissolved oxygen tension control