J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (8): 978-982.

• Research Articles • Previous Articles     Next Articles

Bioaccumulation of Nickel by Various Scenedesmus Species in Culture Solution Containing Nickel

HU Zhang-Li, WONG Yuk-Shan and TAM Fung-Yee   

Abstract:

Sixteen Scenedesmus species or strains have been employed to investigate the maximum capacity of nickel (Ni) accumulation in 10 mg/L Ni solution. The results showed that the capacity of accumulating Ni from aqueous solution in 16 Scenedesmus species or strains showed the diversity.S. quadricauda freshwater algae culture collection of the Institute of Hydrobiology (FACHB) 44 and S. quadricauda FACHB 506 performed much more capacity of Ni accumulation than other species such as Scenedesmus sp. FACHB 416 and Scenedesmus sp. FACHB 489. Sequestration of Ni ions from aqueous solution was very efficient (26.7 mg Ni/g dry weight, in the 100 mg/L Ni solution) in S.quadricauda FACHB 44. The kinetics of Ni binding indicated that Ni bioaccumulation, in algal cell of S. quadricauda FACHB 44, possessed a rapid biosorption (5 min) and an slow bioaccumulation (2-3 h). More than 70% of Ni binding in algal cell were accumulated by biosorption and the remaining 20%-30% were bioaccumulated by energy consumed transportation. It is much more higher ratio of energy consumed transportation in S. quadricauda FACHB 44 than in other algae. Both the transmission electron microscope (TEM) and the energy dispersive X ray (EDX) microanalyses also revealed the different mechanisms of bioaccumulation in the various subcellular regions: a very fast adsorption in the cell wall; and a time dependent absorption in protoplasm, specially in starch and chromatin

栅藻对水环境中镍的累积效应与机理分析
胡章立2黄玉山1* 谭凤仪1

(1. 香港城市大学生物及化学系海岸污染与保护中心,香港;2. 深圳大学生命科学学院,深圳518060)

摘要: 对不同Scenedesmus品种的藻细胞从含镍水溶液 (10mg/L)中累积金属镍的能力进行了分析 ,结果表明 :藻细胞对镍的生物累积量表现出明显的品种差异性。Scenedesmusquadricauda FACHB 4 4和Scenedesmusquadricauda FACHB 5 0 6表现出很强的累积能力 (累积量达到 5~ 6mgNi /g干重 ) ,而Scenedesmus sp .FACHB 4 16和Scenedesmussp .FACHB 4 89在相同条件对金属镍累积量要少得多 (1~ 1.5mgNi /g干重 )。这种差异可能与不同品种藻细胞间的形态结构和生理特性是相关的。对S .quadricaudaFACHB 4 4重金属抗性和累积能力进一步的分析表明 ,S .quadricaudaFACHB 4 4用于含镍重金属废水处理是非常有效的 ,在高浓度 (10 0mg/L)的镍溶液中 ,藻细胞的最大累积量能达到 (2 6 .7mgNi/g干重 )。对该藻细胞镍累积动力学分析发现 :藻细胞对镍的生物累积包括一个快速的被动吸附过程 (5min ,结合 70 %的镍 )和一个缓慢的耗能累积过程 (2~ 3h时间内的累积量占总量的 2 0 %~ 30 % )。与其他藻类相比 ,S .quadricaudaFACHB 4 4对水溶液中镍的耗能累积量明显高于其他藻类。透射电子显微镜(TEM)和X射线能谱 (EDX)分析结果均表明 ,藻细胞耗能累积的镍主要集中在原生质体中 ,尤以淀粉粒和染色质中为多。
 

关键词: 生物累积;栅藻;镍

Key words: bioaccumulation, Scenedesmus, nickel (Ni)

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