J Integr Plant Biol. ›› 2002, Vol. 44 ›› Issue (9): 1011-1021.

• Research Articles •     Next Articles

In Vitro Fertilization of Angiosperms—10 Year Effort in China

SUN Meng Xiang*, YANG Hong Yuan   

  • Published:2002-09-21

Abstract:

This review gives a brief retrospect to the development on in vitro fertilization (IVF) of angiosperms in China. During the last decade Chinese scientists put great enthusiasm and efforts on IVF system construction and builtupnotable contributions to the flourish of this field. Keeping pace with international development and participating international cooperation in the field of IVF, Chinese scientists have now focused on the investigation of basic mechanism relevant to possible gamete interaction, egg cell activation and early embryogenesis by IVF. In vitro manipulation techniques are combined with cytological and molecular biological approaches to unveil the double fertilization mysteries.

我国被子植物体外受精研究——十年回顾
孙蒙祥* 杨弘远
(武汉大学植物发育生物学教育部重点实验室,武汉430072)

摘要: 简要回顾了十余年来我国被子植物体外受精领域的研究历程和主要成就。我国科学家在性细胞的分离,尤其是生活胚囊和雌配子的分离方面走在国际前列。在一定程度上我国科学家的开拓性工作引起了国际同行的广泛兴趣和重视,推动了该领域的迅速发展,为实现被子植物真正意义的体外受精做出了独特的贡献。十年来,我国已建立了自己独特的被子植物离体受精实验技术系统并利用该系统与国际同行合作进行了一系列研究,尤其在探讨配子相互作用、卵细胞激活、避免多精入卵的机制等方面做出了创新性工作,为正确认识体外受精系统的优越性与局限性,也为深入探讨受精机制提供了有价值的新资料。目前正以体外受精操作系统结合细胞生物学和分子生物学等多种手段对受精过程中的一些重要事态与机理做进一步探讨。
关键词: 配子;体外受精;被子植物

通讯作者。E-mail:mxsun @ whu.edu.cn.

Key words: gamete;in vitro fertilization, angiosperm

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