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J Integr Plant Biol, 2003, 45 (11): 1373-1377, Research Article
Probing Lectin Receptors on the Plasma Membrane of Isolated Viable Generative Cells in Angiosperms by Means of Single Cell Manipulation
FANG Ke-Feng, SUN Meng-Xiang*, ZHOU Chang
doi:
Abstract

Fluorescein isothiocyanate (FITC) conjugated concanavalin agglutinin (Con A), wheat germ agglutinin (WGA) and soybean agglutinin (SBA) were used as probes to localize their specific receptors on the plasma membrane of generative cells (GCs) isolated from Vicia faba L., Iris tectorium Maxim. and Hippeastrum vittatum Herb. It is a further investigation on possible distributive dynamic of lectin receptors during the developmental process from generative cells to sperm cells. In the present study, all the three lectin receptors were found on the surface of generative cells of V. faba and I. tectorium. However, on generative cells of H. vittatum only Con A and WGA, but not SBA receptors were observed. The same lectin receptors on the generative cells from different species showed various distribution patterns. The distribution of various lectin receptors on the same generative cells also showed different characteristics. Lectin receptors were totally absent on some generative cells of all three investigated species. Polar distribution of lectin receptors was observed on tailed generative cells. The findings offer important clues to investigate sperm cell function and possible sperm dimorphism of surface glycoprotein.

几种植物生殖细胞质膜表面的凝集素受体荧光标记
房克凤 孙蒙祥  周 嫦
(武汉大学生命科学学院植物发育生物学教育部重点实验室, 武汉 430072)


摘要: 为进一步探讨从生殖细胞到精子的发育过程中细胞质膜表面凝集素受体的可能变化,及其与两类对凝集素标记有不同结果的精子的关系,用异硫氰酸荧光素标记的伴刀豆凝集素(Con A)、麦芽凝集素(WGA)和大豆凝集素(SBA)对蚕豆(Vicia faba L.)、鸢尾(Iris tectorium Maxim.)和朱顶红(Hippeastrum vittatum Herb.)的生殖细胞质膜表面的凝集素受体进行标记。结果显示:在不同植物中均有部分生殖细胞不能被凝集素探针标记,且在保持尾状形态的生殖细胞的表面发现有凝集素受体的极性分布。这可能是导致部分精子表面不能被同种凝集素标记的重要原因。此外,同一种凝集素受体在不同物种的生殖细胞上分布不一致,不同的凝集素受体在同一种植物的生殖细胞上的分布模式亦有不同。在蚕豆和鸢尾的生殖细胞表面均有这三种凝集素的受体。在朱顶红生殖细胞的表面有前两种凝集素的受体,分布比较均一,但是没有大豆凝集素的受体。此外,在具尾生殖细胞表面发现有凝集素受体极性分布的现象,为探讨精细胞功能及其表面糖蛋白分布的可能差异提供了重要启示。
关键词: 生殖细胞;凝集素;蚕豆;鸢尾;朱顶红

通讯作者。 E-mail: <mxsun@whu.edu.cn>。

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