J Integr Plant Biol. ›› 2003, Vol. 45 ›› Issue (12): 1453-1464.

• Research Articles • Previous Articles     Next Articles

Control of In Vitro Regeneration of Individual Reproductive and Vegetative Organs in Dracaena fragrans cv. massangeana Hort.— Regularities of the Direct Regeneration of Individual Organs In Vitro

LU Wen-Liang   


Various individual organs (tepal, flower bud, inflorescence branch, inflorescence, adult vegetative bud and juvenile vegetative bud) were directly regenerated respectively by callus in Dracaena fragrans cv. massangeana Hort. During the regeneration of these individual organs some regularity phenomena were observed. Firstly, the kind range of the individual organs, which are directly regenerated in vitro, is in close relationship to the differentiated stages of the organs used for explant excision during plant ontogeny. The explants excised from the epigeous organ that is differentiated at some stage (stage A) during plant ontogeny must be able to separately regenerate all of those individual epigeous organs: ones differentiated slightly later than the stage A, ones differentiated at the stage A and all ones differentiated earlier than the stage A. Secondly, within this range which kind of organ is regenerated depends on the exogenous auxin concentrations in medium. With the gradual increase of 2,4-D concentration from 0.005 mg/L to 0.5 mg/L, the kinds of regenerated organs will change by the order as follows: vegetative bud, inflorescence, inflorescence branch, flower bud, tepal. These regularities will be able to be used for inducing the direct regeneration of a given epigeous organ in angiosperms.


Key words: direct regeneration of a given organ, regular patterns for the direct regeneration of individual organs, reversed development of cells in vitro , cell totipotency expression, Dracaena fragrans

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