Abstract:

cDNA libraries were constructed from the leaves of a rice salt tolerance variety Tesan’ai 2 growing in solutions with 150 mmol/L NaCl for 3 h or without salt stress. Three salt-responsive cDNA clones, Ts1, Ts2 and Ts3 were isolated by differential screening. Northern blotting analysis showed that the transcription levels of Ts1 and Ts2 increased within 3 h salt stress and kept on increasing within 24 h, while the transcription level of Ts3 reached its peak within 3 h. Sequence analysis indicated that there were no homologies between the three cDNA clones and any known gene. The three cDNA clones were mapped using a doubled haploid (DH) population derived from an indica variety ZYQ8, which was a salt tolerance parent of Tesan’ai 2, with a japonica variety JX17. Ts1, Ts2 and Ts3 were located on chromosomes 1, 3, and 7, respectively. It was noted that Ts1, Ts2, and Ts3 were in or near the regions of major or minor salt tolerance quantitative trait loci (QTLs), which were mapped in the same DH population in a parallel study.

水稻盐胁迫应答c D N A 的克隆、表达和染色体定位
钱 前 柳原城司 滕 胜  曾大力 朱立煌  陈受宜

(1.中国科学院遗传与发育生物学研究所，北京 100101；2.中国水稻研究所农业部水稻生物学重点实验室，杭州 310006; 3. Okinawa Subtropical Station, Japan International Research Center for Agricultural Sciences, Ishigaki 907, Okinawa, Japan)

摘要： 从150 mmol/L 盐胁迫3 h 和没有胁迫的耐盐水稻(Oryza sativa L.)品种特三矮2 号叶片中提取mRNA，构建cDNA 文库。通过示差筛选，得到3 个盐胁迫应答cDNA 克隆Ts1、Ts2 和Ts3。Northern 分析表明，3 h 的盐胁迫可使Ts1、Ts2 和Ts3 的转录水平明显上升；3~24 h 期间，Ts1 和Ts2 的转录水平继续上升，而Ts3 的转录水平则下降。序列分析表明，这3 个cDNA 克隆与已知功能的基因没有同源性。利用特三矮2 号的耐盐亲本ZYQ8 和粳稻JX17 组合构建了DH 群体和分子标记连锁图谱，将Ts1、Ts2 和Ts3 分别定位在第1、3 和7 染色体上。值得注意的是，Ts1、Ts2 和Ts3 与用同一群体定位的主效和微效耐盐QTL 位于同一或相邻区域。
关键词： 水稻；示查筛选；盐胁迫应答基因；定位

通讯作者。Tel:010-64886859；E-mail:<sychen@genetics.ac.cn>。