J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (10): 1135-1140.

• Research Articles •     Next Articles

Cloning and Sequencing the g Subunit of R-Phycoerythrin from Corallina officinalis

WANG Sheng, ZHONG Fu-Di, WU Zu-Jian, LIN Qi-Ying, XIE Lian-Hui   


The full-length cDNA of the g subunit of R-phycoerythrin from Corallina officinalis L. was cloned by rapid amplification of cDNA ends (RACE) method, and sequenced. The full-length cDNA is a 2 308 bp consisting of 5'' untranslated region (UTR) of 1 203 bp, an open reading frame (ORF) of 960 bp that encodes 320 amino acids, and 3'' UTR of 145 bp. The mature g polypeptide contains two unique internal repeat domains as reported by Apt et al. (2001). Sequence analysis of the different clones revealed different 3''-end sequences at the g subunit. The difference between the 3''-end sequences suggests that the g subunit may have more than one copy, or have gone through different post-transcriptional modification. By comparing the DNA and cDNA sequences, we found that the g subunit is an intronless gene. This is the first report of the g subunit gene of R-phycoerythrin from C. officinalis .

Key words: Corallina officinalis , R-phycoerythrin, g subunit, rapid amplification of cDNA ends (RACE)

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