J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (10): 1186-1191.

• Research Articles • Previous Articles     Next Articles

Isolation and Characterization of the Cytoplasmic Membrane from the Terrestrial Cyanobacterium—Nostoc flagelliforme

HUANG Hui, ZHONG Ze-Pu, WANG Ke-Bin, BAI Ke-Zhi, LI Liang-Bi, KUANG Ting-Yun   


Cytoplasmic membrane of Nostoc flagelliforme Born. et Flah. was isolated for the first time with a new method, the unique feature of which is the combined use of French pressure cell and digitonin to disrupt cells. After passed twice through French pressure cell (at 80 MPa), cells in sample (20 mg of dry weight/mL) were disrupted effectively by digitonin (3 mg/mL), and then the cytoplasmic membrane was isolated by density gradient centrifugation. The membrane contained carotenoids with absorption peaks at 458, 487 and 524 nm and a precursor of chlorophyll a with a minor peak at 673 nm. The fluorescence emission peaks of the membrane were emitted from the precursor of chlorophyll a. More than 30 polypeptides were detected in the membrane, in which the most obvious corresponded to the polypeptides with molecular mass of 80, 28, 19 and 17 kD. The membrane contained four types of glycerolipids: MGDG (62.4%), DGDG (18.9%), SQDG (16.7%) and PG (2.0%). 16:0, 16:1 [9], 18:0, 18:1 [9], 18:2 [9, 12] and 18:3 [9, 12, 15] fatty acids were determined in the membrane, in which 16:1 and 18:3 fatty acids were the main components, representing 32.3% and 34.4% of the total fatty acids respectively. High proportion of 18:3 fatty acid in the cytoplasmic membrane may be an important factor of N. flagelliforme in its remarkable drought-tolerant ability.

Key words: Nostoc flagelliforme , cytoplasmic membrane, isolation, digitonin

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