J Integr Plant Biol. ›› 2006, Vol. 48 ›› Issue (11): -.DOI: 10.1111/j.1744-7909.2006.00325.x

• Research Articles •    

Isolation and Structural Analysis of an Acidic Polysaccharide from Astragalus membranaceus (Fisch.) Bunge

Shun-Chun Wang, Jun-Jie Shan, Zheng-Tao Wang and Zhi-Bi Hu   

Abstract: A new water-soluble hetero-polysaccharide, APSID3, was obtained from a hot-water extract of the roots of Astragalus membranaceus (Fisch.) Bunge by DEAE-Sepharose Fast Flow and Sephacryl S-300 chromatography. The molecular weight of APSID3 was estimated to be 5.79 × 105 Da. Based on a sugar composition analysis, methylation analysis, partial hydrolysis and 13C nuclear magnetic resonance experimentation, it was concluded that the minimal repeat unit of APSID3 was composed of one terminal arabinose, one 1,5-linked arabinose, one 1,3-linked rhamnose, one 1,3,4-linked rhamnose, five 1,4-linked methyl galacturonates and six 1,4-linked methyl glucuronates.(Author for correspondence. Tel: +86 (0)21 5132 2507; E-mail: zhibihu@hotmail.com)

Key words: Astragalus membranaceus, hetero-polysaccharide, isolation and purification, structural analysis.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22