J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (10): 1192-1199.

• Research Articles • Previous Articles     Next Articles

Characterization of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Green Alga, Bryopsis corticulans

CHEN Hui, SHEN Shi-Hua, GONG Yan-Dao, HE Jun-Fang, WANG Guang-Ce, LI Liang-Bi, KUANG Ting-Yun   

Abstract:

The main light-harvesting chlorophyll a/b -protein complex (LHCⅡ) has been isolated directly from thylakoid membranes of shiphonous green alga, Bryopsis corticulans Setch. by using two consecutive runs of anion exchange and gel-filtration chromatography. Monomeric and trimeric subcomplexes of LHCⅡ were obtained by using sucrose gradient ultracentrifugation. Pigment analysis by reversed-phase high performance liquid chromatography showed that chlorophyll a (Chl a), chlorophyll b (Chl b), neoxanthin, violaxanthin and siphonaxanthin were involved in LHCⅡ from B. corticulans. The properties of electronic transition of monomeric LHCⅡ showed similarities to those of trimeric LHCⅡ. Circular dichroism spectroscopy showed that strong intramolecular interaction of excitonic dipoles between Chl a and between Chl b exist in one LHCⅡ apoprotein, while the intermolecular interaction of these dipoles can be intensified in the trimeric structure. The monomer has high efficient energy transfer from Chl b and siphonaxanthin to Chl a similarly to that of the trimer. Our results suggest that in B. corticulans, LHCⅡ monomer has high ordered pigment organization that play effective physiological function as the trimer, and thus it might be also a functional organization existing in thylakoid membrane of B. corticulans.

Key words: light-harvesting protein complex, monomer, pigment composition, pigment interaction, Bryopsis corticulans

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