J Integr Plant Biol. ›› 2004, Vol. 46 ›› Issue (4): 436-442.

• Research Articles • Previous Articles     Next Articles

Fluorescent In Situ Hybridization Analysis of Rye Chromatin in the Background of “Xiaoyan No.6”

WANG Zhi-Guo, AN Tiao-Guo, LI Jun-Ming, Molnar-Lang MARTA JI Jun, ZHONG Guan-Chang, MU Su-Mei   

Abstract:

A set of wheat germplasms with rye’s characters was obtained by crossing the Triticum aestivum L. cv. “Xiaoyan No.6” and Secale cereale L. cv. “German White”. Eight lines were found carrying rye chromosomes or segments by genomic in situ hybridization (GISH), including three addition lines, one substitution line and four translocation lines. Two-color fluorescent in situ hybridization (FISH) was used to identify the genome composition and individual chromosome of the hybrids using pSc119.2 labled with fluorogreen and pAs1 labled with fluorored as probes. The partial results showed that BC116-1 is a wheat-rye 1RS/1BL translocation line; BC152 is a monosomic 1RS/1BL translocation line; BC97-2 is a disomic substitution line with a pair of 2D chromosomes substituted by 2R; BC122-3 is a 6R monosomic addition line with one of the long arm of 6B deletion. The application of sequential GISH and two-color FISH in wheat breeding is discussed.

Key words: rye chromatin, Xiaoyan No.6, fluoroscent in situ hybridization

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