J Integr Plant Biol. ›› 2018, Vol. 60 ›› Issue (5): 369-375.DOI: 10.1111/jipb.12620

Special Issue: CRISPR

• Letters to the Editor • Previous Articles     Next Articles

Generation of new glutinous rice by CRISPR/Cas9‐targeted mutagenesis of the Waxy gene in elite rice varieties

Jinshan Zhang1,2†, Hui Zhang1,3†*, José Ramón Botella4 and Jian-Kang Zhu1,3*   

  1. 1Shanghai Center for Plant Stress Biology, and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China
    2University of Chinese Academy of Sciences, Shanghai 201602, China
    3Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA
    4Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane QLD 4072, Australia
  • Received:2017-11-23 Accepted:2017-12-01 Online:2017-12-06 Published:2018-05-10
  • About author:These authors contributed equally to this work.
    **Correspondences: Email: Hui Zhang (huizhang@sibs.ac.cn); Jian-Kang Zhu (jkzhu@sibs.ac.cn, Dr. Zhu is fully responsible for the distribution of all materials associated with this article)


In rice, amylose content (AC) is controlled by a single dominant Waxy gene. We used Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR‐associated 9 (Cas9) to introduce a loss‐of‐function mutation into the Waxy gene in two widely cultivated elite japonica varieties. Our results show that mutations in the Waxy gene reduce AC and convert the rice into glutinous ones without affecting other desirable agronomic traits, offering an effective and easy strategy to improve glutinosity in elite varieties. Importantly, we successfully removed the transgenes from the progeny. Our study provides an example of generating improved crops with potential for commercialization, by editing a gene of interest directly in elite crop varieties.

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