BBX9 forms feedback loops with PIFs and BBX21 to promote photomorphogenic development

doi:10.1111/jipb.13746

J Integr Plant Biol. ›› 2024, Vol. 66 ›› Issue (9): 1934-1952.DOI: 10.1111/jipb.13746 cstr: 32098.14.jipb.13746

• Cell and Developmental Biology • Previous Articles Next Articles

BBX9 forms feedback loops with PIFs and BBX21 to promote photomorphogenic development

Zhaoqing Song¹, Wanying Ye¹, Qing Jiang¹, Huan Lin¹, Qing Hu¹, Yuntao Xiao¹, Yeting Bian¹, Fengyue Zhao¹, Jie Dong² and Dongqing Xu^1*

1. State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, National Center for Soybean Improvement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
2. Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
^*Correspondence: Dongqing Xu (dongqingxu@njau.edu.cn)

Received:2023-08-08 Accepted:2024-07-05 Online:2024-07-23 Published:2024-09-01
Supported by:
This work was supported by the Natural Science Foundation of Jiangsu for Distinguished Young Scholars (BK20211525), the Fundamental Research Funds for the Central Universities (YDZX2024045), the National Natural Science Foundation of China (32270256), the Postdoctoral Fellowship Program of CPSF (GZB20240319), the Core Technology Development for Breeding Program of Jiangsu Province (JBGS‐2021‐014) and the Jiangsu Collaborative Innovation Center for Modern Crop Production (to D.X.)

Abstract

Abstract: Light is one of the most essential environmental factors that tightly and precisely control various physiological and developmental processes in plants. B-box CONTAINING PROTEINs (BBXs) play central roles in the regulation of light-dependent development. In this study, we report that BBX9 is a positive regulator of light signaling. BBX9 interacts with the red light photoreceptor PHYTOCHROME B (phyB) and transcription factors PHYTOCHROME-INTERACTING FACTORs (PIFs). phyB promotes the stabilization of BBX9 in light, while BBX9 inhibits the transcriptional activation activity of PIFs. In turn, PIFs directly bind to the promoter of BBX9 to repress its transcription. On the other hand, BBX9 associates with the positive regulator of light signaling, BBX21, and enhances its biochemical activity. BBX21 associates with the promoter regions of BBX9 and transcriptionally up-regulates its expression. Collectively, this study unveiled that BBX9 forms a negative feedback loop with PIFs and a positive one with BBX21 to ensure that plants adapt to fluctuating light conditions.

Key words: Arabidopsis, BBX21, BBX9, light signaling, photo-morphogenesis, phyB, PIF

Zhaoqing Song, Wanying Ye, Qing Jiang, Huan Lin, Qing Hu, Yuntao Xiao, Yeting Bian, Fengyue Zhao, Jie Dong and Dongqing Xu. BBX9 forms feedback loops with PIFs and BBX21 to promote photomorphogenic development[J]. J Integr Plant Biol., 2024, 66(9): 1934-1952.

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BBX9 forms feedback loops with PIFs and BBX21 to promote photomorphogenic development

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